BMRB Entry 16797

Name: Structure of the three-Cys2His2 domain of mouse testis zinc finger protein
Published: 2010-07-27

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PDB ID: 2kvh
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16797
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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Structure of the three-Cys2His2 domain of mouse testis zinc finger protein

Deposition date:

2010-03-29

Original release date:

2010-07-27

Authors:

Chou, Chun-Chi; Lou, Yuan-Chao; Chen, Chinpan

Citation:

Citation: Chou, Chun-Chi; Lou, Yuan-Chao; Tang, Tang; Chen, Chinpan. "Structure and DNA binding characteristics of the three-Cys2His2 domain of mouse testis zinc finger protein." Proteins 78, 2202-2212 (2010).
PubMed: 20544958

Assembly members:

Assembly members:
mTZD_finger2, polymer, 27 residues, 3120.5 Da.
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETBlue-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
mTZD_finger2: EKPFSCSLCPQRSRDFSAMT KHLRTHG

Data sets:

assigned_chemical_shifts
- assignments_zf2

Data type	Count
13C chemical shifts	106
15N chemical shifts	26
1H chemical shifts	175

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	mTZD finger2	1
2	ZINC ION	2

Entities:

Entity 1, mTZD finger2 27 residues - 3120.5 Da.

1

GLU

LYS

PRO

PHE

SER

CYS

SER

LEU

CYS

PRO

2

GLN

ARG

SER

ARG

ASP

PHE

SER

ALA

MET

THR

3

LYS

HIS

LEU

ARG

THR

HIS

GLY

Entity 2, ZINC ION - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: mTZD finger2 mM; acetic acid, [U-99% 2H], 50 mM; sodium chloride 20 mM

sample_conditions_1: ionic strength: 20 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1

Software:

X-PLOR NIH v2.18, Schwieters, Kuszewski, Tjandra and Clore - structure solution

NMRView v5, Johnson, One Moon Scientific - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

TALOS, Cornilescu, Delaglio and Bax - data analysis

NMR spectrometers:

Bruker Avance 800 MHz
Bruker Avance 600 MHz

PDB	2KVH
DBJ	BAB30015 BAG73879
GB	AAD27708 AAD48448 AAF61244 AAI37688 AAK13198
REF	NP_001102987 NP_001129098 NP_001178153 NP_001303831 NP_001303832
SP	A1YGK1 A2T7E6 Q9JKD9 Q9Y2Y4
TPG	DAA19797
AlphaFold	A1YGK1 A2T7E6 Q9JKD9 Q9Y2Y4