BMRB Entry 16788

Name: Solution structure of NusE:NusG-CTD complex
Published: 2011-05-02

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PDB ID: 2kvq
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16788
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of NusE:NusG-CTD complex

Deposition date:

2010-03-25

Original release date:

2011-05-02

Authors:

Burmann, Bjoern; Schweimer, Kristian; Roesch, Paul

Citation:

Citation: Burmann, Bjoern; Schweimer, Kristian; Luo, Xiao; Wahl, Markus; Stitt, Barbara; Gottesman, Max; Roesch, Paul. "A NusE:NusG Complex Links Transcription and Translation" Science 328, 501-504 (2010).
PubMed: 20413501

Assembly members:

Assembly members:
NusE, polymer, 87 residues, 8937.450 Da.
NusG-CTD, polymer, 63 residues, 6639.524 Da.

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-6P

Entity Sequences (FASTA):

Entity Sequences (FASTA):
NusE: GPLGSMQNQRIRIRLKAFDH RLIDQATAEIVETAKRTGAQ VRGPIPLPTRSRTHLRLVDI VEPTEKTVDALMRLDLAAGV DVQISLG
NusG-CTD: GAMGRPKTLFEPGEMVRVND GPFADFNGVVEEVDYEKSRL KVSVSIFGRATPVELDFSQV EKA

Data sets:

assigned_chemical_shifts
- Assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	410
15N chemical shifts	120
1H chemical shifts	928

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	NusE	1
2	NusG	2

Entities:

Entity 1, NusE 87 residues - 8937.450 Da.

1

GLY

PRO

LEU

GLY

SER

MET

GLN

ASN

GLN

ARG

2

ILE

ARG

ILE

ARG

LEU

LYS

ALA

PHE

ASP

HIS

3

ARG

LEU

ILE

ASP

GLN

ALA

THR

ALA

GLU

ILE

4

VAL

GLU

THR

ALA

LYS

ARG

THR

GLY

ALA

GLN

5

VAL

ARG

GLY

PRO

ILE

PRO

LEU

PRO

THR

ARG

6

SER

ARG

THR

HIS

LEU

ARG

LEU

VAL

ASP

ILE

7

VAL

GLU

PRO

THR

GLU

LYS

THR

VAL

ASP

ALA

8

LEU

MET

ARG

LEU

ASP

LEU

ALA

GLY

VAL

9

ASP

VAL

GLN

ILE

SER

LEU

GLY

Entity 2, NusG 63 residues - 6639.524 Da.

1

GLY

ALA

MET

GLY

ARG

PRO

LYS

THR

LEU

PHE

2

GLU

PRO

GLY

GLU

MET

VAL

ARG

VAL

ASN

ASP

3

GLY

PRO

PHE

ALA

ASP

PHE

ASN

GLY

VAL

4

GLU

VAL

ASP

TYR

GLU

LYS

SER

ARG

LEU

5

LYS

VAL

SER

VAL

SER

ILE

PHE

GLY

ARG

ALA

6

THR

PRO

VAL

GLU

LEU

ASP

PHE

SER

GLN

VAL

7

GLU

LYS

ALA

Samples:

15N_13C_-NusG-CTD_NusE_NusB: 15N, 13C -NusG-CTD, [U-100% 13C; U-100% 15N], 300 uM; NusE 300 uM; NusB 300 uM; H2O 90%; D2O 10%; Sodium Chloride 50 mM; HEPES 25 mM

15N_13C_2H-NusE_NusB_NusG-CTD: 15N, 13C, 2H-NusE, [U-100% 13C; U-100% 15N; U-80% 2H], 300 uM; NusG-CTD 300 uM; NusB 300 uM; H2O 90%; D2O 10%; Sodium Chloride 50 mM; HEPES 25 mM

NusG-CTD_15N_13C_NusE_NusB: NusG-CTD 0.3 mM; 15N, 13C NusE, [U-100% 13C; U-100% 15N], 0.3 mM; NusB 0.3 mM; H2O 90%; D2O 10%; Sodium Chloride 50 mM; HEPES 25 mM

sample_conditions_1: ionic strength: 75 mM; pH: 7.5; temperature: 310 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	15N_13C_-NusG-CTD_NusE_NusB	isotropic	sample_conditions_1
2D 1H-13C HSQC	15N_13C_-NusG-CTD_NusE_NusB	isotropic	sample_conditions_1
3D 1H-15N NOESY	15N_13C_-NusG-CTD_NusE_NusB	isotropic	sample_conditions_1
3D 1H-13C NOESY	15N_13C_-NusG-CTD_NusE_NusB	isotropic	sample_conditions_1
3D 13C filtered(F1)-13C edited NOESY	15N_13C_-NusG-CTD_NusE_NusB	isotropic	sample_conditions_1
2D 1H-15N HSQC	NusG-CTD_15N_13C_NusE_NusB	isotropic	sample_conditions_1
2D 1H-13C HSQC	NusG-CTD_15N_13C_NusE_NusB	isotropic	sample_conditions_1
3D 1H-13C NOESY	NusG-CTD_15N_13C_NusE_NusB	isotropic	sample_conditions_1
3D 1H-15N NOESY	NusG-CTD_15N_13C_NusE_NusB	isotropic	sample_conditions_1
3D 13C filtered(F1)-133D 13C filtered(F1)-13C edited NOESY	NusG-CTD_15N_13C_NusE_NusB	isotropic	sample_conditions_1
3D HNCA	15N_13C_2H-NusE_NusB_NusG-CTD	isotropic	sample_conditions_1
3D HNCACB	15N_13C_2H-NusE_NusB_NusG-CTD	isotropic	sample_conditions_1
3D HCCH-TOCSY	NusG-CTD_15N_13C_NusE_NusB	isotropic	sample_conditions_1
3D HCCH-TOCSY	15N_13C_-NusG-CTD_NusE_NusB	isotropic	sample_conditions_1

Software:

xwinnmr, Bruker Biospin - collection

NMRView, Johnson, One Moon Scientific - chemical shift assignment, peak picking

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution

NMR spectrometers:

Bruker Avance 700 MHz
Bruker Avance 800 MHz

PDB	2KVQ 3D3B 3D3C 3IMQ 2JVV 2KVQ
EMBL	CDK48609 CDK60294 CDL02401 CDL28926 CDL47417 CAD09492 CAP78438 CAQ34328 CAQ91223 CAR00955
GB	ESD90132 AAA24622 AAC43080 AAC76956 AAF33495 AAG59178
BMRB	15490
DBJ	BAB38328 BAE77338 BAG79793 BAH61119 BAI28242
PIR	AB0934
REF	NP_290613 NP_312932 NP_418409 NP_457922 NP_463017
SP	P0AA01 P0AA02 P0AA03 P0AFG0 P0AFG1
AlphaFold	P0AA01 P0AA02 P0AA03 P0AFG0 P0AFG1