BMRB Entry 16788

Title:
Solution structure of NusE:NusG-CTD complex   PubMed: 20413501
Deposition date:
2010-03-25
Original release date:
2011-05-02
Authors:
Burmann, Bjoern; Schweimer, Kristian; Roesch, Paul
Citation:

Citation: Burmann, Bjoern; Schweimer, Kristian; Luo, Xiao; Wahl, Markus; Stitt, Barbara; Gottesman, Max; Roesch, Paul. "A NusE:NusG Complex Links Transcription and Translation"  Science 328, 501-504 (2010).

Assembly members:

Assembly members:
NusE, polymer, 87 residues, 8937.450 Da.
NusG-CTD, polymer, 63 residues, 6639.524 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-6P

Experimental source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-6P

Data sets:
Data typeCount
13C chemical shifts410
15N chemical shifts120
1H chemical shifts928

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1NusE1
2NusG2

Entities:

Entity 1, NusE 87 residues - 8937.450 Da.

1   GLYPROLEUGLYSERMETGLNASNGLNARG
2   ILEARGILEARGLEULYSALAPHEASPHIS
3   ARGLEUILEASPGLNALATHRALAGLUILE
4   VALGLUTHRALALYSARGTHRGLYALAGLN
5   VALARGGLYPROILEPROLEUPROTHRARG
6   SERARGTHRHISLEUARGLEUVALASPILE
7   VALGLUPROTHRGLULYSTHRVALASPALA
8   LEUMETARGLEUASPLEUALAALAGLYVAL
9   ASPVALGLNILESERLEUGLY

Entity 2, NusG 63 residues - 6639.524 Da.

1   GLYALAMETGLYARGPROLYSTHRLEUPHE
2   GLUPROGLYGLUMETVALARGVALASNASP
3   GLYPROPHEALAASPPHEASNGLYVALVAL
4   GLUGLUVALASPTYRGLULYSSERARGLEU
5   LYSVALSERVALSERILEPHEGLYARGALA
6   THRPROVALGLULEUASPPHESERGLNVAL
7   GLULYSALA

Samples:

15N_13C_-NusG-CTD_NusE_NusB: 15N, 13C -NusG-CTD, [U-100% 13C; U-100% 15N], 300 uM; NusE 300 uM; NusB 300 uM; H2O 90%; D2O 10%; Sodium Chloride 50 mM; HEPES 25 mM

15N_13C_2H-NusE_NusB_NusG-CTD: 15N, 13C, 2H-NusE, [U-100% 13C; U-100% 15N; U-80% 2H], 300 uM; NusG-CTD 300 uM; NusB 300 uM; H2O 90%; D2O 10%; Sodium Chloride 50 mM; HEPES 25 mM

NusG-CTD_15N_13C_NusE_NusB: NusG-CTD 0.3 mM; 15N, 13C NusE, [U-100% 13C; U-100% 15N], 0.3 mM; NusB 0.3 mM; H2O 90%; D2O 10%; Sodium Chloride 50 mM; HEPES 25 mM

sample_conditions_1: ionic strength: 75 mM; pH: 7.5; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQC15N_13C_-NusG-CTD_NusE_NusBisotropicsample_conditions_1
2D 1H-13C HSQC15N_13C_-NusG-CTD_NusE_NusBisotropicsample_conditions_1
3D 1H-15N NOESY15N_13C_-NusG-CTD_NusE_NusBisotropicsample_conditions_1
3D 1H-13C NOESY15N_13C_-NusG-CTD_NusE_NusBisotropicsample_conditions_1
3D 13C filtered(F1)-13C edited NOESY15N_13C_-NusG-CTD_NusE_NusBisotropicsample_conditions_1
2D 1H-15N HSQCNusG-CTD_15N_13C_NusE_NusBisotropicsample_conditions_1
2D 1H-13C HSQCNusG-CTD_15N_13C_NusE_NusBisotropicsample_conditions_1
3D 1H-13C NOESYNusG-CTD_15N_13C_NusE_NusBisotropicsample_conditions_1
3D 1H-15N NOESYNusG-CTD_15N_13C_NusE_NusBisotropicsample_conditions_1
3D 13C filtered(F1)-133D 13C filtered(F1)-13C edited NOESYNusG-CTD_15N_13C_NusE_NusBisotropicsample_conditions_1
3D HNCA15N_13C_2H-NusE_NusB_NusG-CTDisotropicsample_conditions_1
3D HNCACB15N_13C_2H-NusE_NusB_NusG-CTDisotropicsample_conditions_1
3D HCCH-TOCSYNusG-CTD_15N_13C_NusE_NusBisotropicsample_conditions_1
3D HCCH-TOCSY15N_13C_-NusG-CTD_NusE_NusBisotropicsample_conditions_1

Software:

xwinnmr, Bruker Biospin - collection

NMRView, Johnson, One Moon Scientific - chemical shift assignment, peak picking

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution

NMR spectrometers:

  • Bruker Avance 700 MHz
  • Bruker Avance 800 MHz

Related Database Links:

PDB
EMBL CDK48609 CDK60294 CDL02401 CDL28926 CDL47417 CAD09492 CAP78438 CAQ34328 CAQ91223 CAR00955
GB ESD90132 AAA24622 AAC43080 AAC76956 AAF33495 AAG59178
BMRB 15490
DBJ BAB38328 BAE77338 BAG79793 BAH61119 BAI28242
PIR AB0934
REF NP_290613 NP_312932 NP_418409 NP_457922 NP_463017
SP P0AA01 P0AA02 P0AA03 P0AFG0 P0AFG1
AlphaFold P0AA02

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts