BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 16780

Title: 1H, 15N, 13C resonance assignments for CobR

Deposition date: 2010-03-19 Original release date: 2014-03-04

Authors: Lawrence, Andrew; Taylor, Samantha; Howard, Mark; Warren, Martin

Citation: Lawrence, Andrew; Taylor, Samantha; Scott, Alan; Pickersgill, Richard; Howard, Mark; Warren, Martin. "Biophysical characterisation of CobR"  .

Assembly members:
CobR, polymer, 173 residues, Formula weight is not available
FAD, non-polymer, 785.550 Da.

Natural source:   Common Name: Brucella melitensis   Taxonomy ID: 29459   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Brucella melitensis

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-14b

Entity Sequences (FASTA):
CobR: MQTVNNIISSVSTVESKAYR DAMSHYAGAVQIVTTAGAAG RRGLTLTAACSVSDNPPTIL ICLQKIHEENRIFIENGVFA INTLAGPHQQLADAFSGRIG LTQDERFELAAWEILATGAP VLKGALAAFDCRVVSVQDHS THHVLFGEVVGLSSHAEEEA LIYLNRRYHKLEL

Data sets:
Data typeCount
13C chemical shifts467
15N chemical shifts158
1H chemical shifts268

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CobR1
2FAD2

Entities:

Entity 1, CobR 173 residues - Formula weight is not available

1   METGLNTHRVALASNASNILEILESERSER
2   VALSERTHRVALGLUSERLYSALATYRARG
3   ASPALAMETSERHISTYRALAGLYALAVAL
4   GLNILEVALTHRTHRALAGLYALAALAGLY
5   ARGARGGLYLEUTHRLEUTHRALAALACYS
6   SERVALSERASPASNPROPROTHRILELEU
7   ILECYSLEUGLNLYSILEHISGLUGLUASN
8   ARGILEPHEILEGLUASNGLYVALPHEALA
9   ILEASNTHRLEUALAGLYPROHISGLNGLN
10   LEUALAASPALAPHESERGLYARGILEGLY
11   LEUTHRGLNASPGLUARGPHEGLULEUALA
12   ALATRPGLUILELEUALATHRGLYALAPRO
13   VALLEULYSGLYALALEUALAALAPHEASP
14   CYSARGVALVALSERVALGLNASPHISSER
15   THRHISHISVALLEUPHEGLYGLUVALVAL
16   GLYLEUSERSERHISALAGLUGLUGLUALA
17   LEUILETYRLEUASNARGARGTYRHISLYS
18   LEUGLULEU

Entity 2, FAD - C27 H33 N9 O15 P2 - 785.550 Da.

1   FAD

Samples:

sample_1: CobR, [U-99% 13C; U-99% 15N], 1 mM; potassium phosphate 20 mM; sodium chloride 100 mM; FAD 1.2 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 100 mM; pH: 6.5; pressure: 1 atm; temperature: 333 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

VNMR, Varian - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

Analysis, CCPN - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

UNP Q8YHT7

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts