BMRB Entry 16768

Click here to enlarge.

PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16768
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
EGF
Deposition date:
2010-03-08
Original release date:
2011-11-28
Authors:
Hsiao Wen, Huang; Mohan, Sepuru; Chin, Yu
Citation:

Citation: Huang, Hsiao Wen; Mohan, Sepuru; Yu, Chin. "The NMR solution structure of human epidermal growth factor (hEGF) at physiological pH and its interactions with suramin"  Biochem. Biophys. Res. Commun. 402, 705-710 (2010).
PubMed: 21029725

Assembly members:

Assembly members:
hEGF, polymer, 53 residues, 6229.053 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-32a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
hEGF: NSDSECPLSHDGYCLHDGVC MYIEALDKYACNCVVGYIGE RCQYRDLKWWELR

Data sets:
Data typeCount
13C chemical shifts184
15N chemical shifts48
1H chemical shifts262

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1hEGF1

Entities:

Entity 1, hEGF 53 residues - 6229.053 Da.

1   ASNSERASPSERGLUCYSPROLEUSERHIS
2   ASPGLYTYRCYSLEUHISASPGLYVALCYS
3   METTYRILEGLUALALEUASPLYSTYRALA
4   CYSASNCYSVALVALGLYTYRILEGLYGLU
5   ARGCYSGLNTYRARGASPLEULYSTRPTRP
6   GLULEUARG

Samples:

sample_1: entity, [U-100% 13C; U-100% 15N], 1 mM; sodium phosphate 50 mM; sodium chloride 20 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.07 M; pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

ARIA v1.1, Dr. Michael Nilges, Institut Pasteur - Automated NOE assignment, NMR structure calculation

NMR spectrometers:

  • Varian Uniform NMR System 700 MHz

Related Database Links:

BMRB 1377 1378 2075 2076 2077 2078
PDB
DBJ BAG61319
EMBL CAA28240 CCA96179 CEK41095
GB AAA60744 AAA72173 AAA72241 AAA72506 AAA72563
REF NP_001171601 NP_001171602 NP_001954 XP_001088957 XP_002804220
SP P01133
AlphaFold P01133

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks