BMRB Entry 16762
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16762
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NMR-STAR v3 text file.
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Citation: Venkitaramani, Deepa; Fulton, D.; Andreotti, Amy; Johansen, Kristen; Johansen, Jorgen. "Mapping the Ca2+-dependent binding of an invertebrate homolog of protein phosphatase 4 regulatory subunit 2 of the small EF-hand protein, calsensin" Biochim. Biophys. Acta 1763, 322-329 (2006).
PubMed: 16600403
Assembly members:
PP4R2, polymer, 423 residues, Formula weight is not available
Calsensin, polymer, 83 residues, Formula weight is not available
Natural source: Common Name: segemented worms Taxonomy ID: 38567 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Hirudo marmorata
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX4T3
Entity Sequences (FASTA):
PP4R2: MGSRKEIRDALEEFLKQPED
GEVNGILERYLSRVAVTGIT
LYPWSKLKPLITRKMNLVVE
EFMKECPMESLALNKNVENT
SVEDMKKRLNDSIELFNGIP
FTIQRLCELLTDQNISKQYR
RVDKFMRGIEKNLNVVTTVD
PFGNRIVGDPKSASNGVLSA
EDDCLLTPTSCTTVKTPPSW
FSSLSSVVNSPDSYPNWKQS
QDEQQQQEQRDAAISSSRER
KKPRSEDDAVDKDCLPPPSK
LFRPGDEFVDEAKQPETKDG
SDSSVATNSTFTPLLTQLLN
GSTPISSSEKEQSVVGSCDS
TESSQPSGLLEILTGKLKDP
ETNLDASEGTPSVDVGAVGT
TSQTDASEVFGAVKSEATQS
TQYHVEITEHANKKDVSTAS
DKKTDEKIQQEPAEVKRTQE
GELESSGVKCQQPSDDPDSL
FSF
Calsensin: MACKVKAELEAAFKKLDANG
DGYVTALELQTFMVTLDAYK
ALSKDKVKEASAKLIKMADK
NSDGKISKEEFLNANAELLC
QLK
- binding_constants
| Data type | Count |
| binding constants | 1 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | PP4R2 | 1 |
| 2 | Calsensin | 2 |
Entities:
Entity 1, PP4R2 423 residues - Formula weight is not available
| 1 | MET | GLY | SER | ARG | LYS | GLU | ILE | ARG | ASP | ALA | ||||
| 2 | LEU | GLU | GLU | PHE | LEU | LYS | GLN | PRO | GLU | ASP | ||||
| 3 | GLY | GLU | VAL | ASN | GLY | ILE | LEU | GLU | ARG | TYR | ||||
| 4 | LEU | SER | ARG | VAL | ALA | VAL | THR | GLY | ILE | THR | ||||
| 5 | LEU | TYR | PRO | TRP | SER | LYS | LEU | LYS | PRO | LEU | ||||
| 6 | ILE | THR | ARG | LYS | MET | ASN | LEU | VAL | VAL | GLU | ||||
| 7 | GLU | PHE | MET | LYS | GLU | CYS | PRO | MET | GLU | SER | ||||
| 8 | LEU | ALA | LEU | ASN | LYS | ASN | VAL | GLU | ASN | THR | ||||
| 9 | SER | VAL | GLU | ASP | MET | LYS | LYS | ARG | LEU | ASN | ||||
| 10 | ASP | SER | ILE | GLU | LEU | PHE | ASN | GLY | ILE | PRO | ||||
| 11 | PHE | THR | ILE | GLN | ARG | LEU | CYS | GLU | LEU | LEU | ||||
| 12 | THR | ASP | GLN | ASN | ILE | SER | LYS | GLN | TYR | ARG | ||||
| 13 | ARG | VAL | ASP | LYS | PHE | MET | ARG | GLY | ILE | GLU | ||||
| 14 | LYS | ASN | LEU | ASN | VAL | VAL | THR | THR | VAL | ASP | ||||
| 15 | PRO | PHE | GLY | ASN | ARG | ILE | VAL | GLY | ASP | PRO | ||||
| 16 | LYS | SER | ALA | SER | ASN | GLY | VAL | LEU | SER | ALA | ||||
| 17 | GLU | ASP | ASP | CYS | LEU | LEU | THR | PRO | THR | SER | ||||
| 18 | CYS | THR | THR | VAL | LYS | THR | PRO | PRO | SER | TRP | ||||
| 19 | PHE | SER | SER | LEU | SER | SER | VAL | VAL | ASN | SER | ||||
| 20 | PRO | ASP | SER | TYR | PRO | ASN | TRP | LYS | GLN | SER | ||||
| 21 | GLN | ASP | GLU | GLN | GLN | GLN | GLN | GLU | GLN | ARG | ||||
| 22 | ASP | ALA | ALA | ILE | SER | SER | SER | ARG | GLU | ARG | ||||
| 23 | LYS | LYS | PRO | ARG | SER | GLU | ASP | ASP | ALA | VAL | ||||
| 24 | ASP | LYS | ASP | CYS | LEU | PRO | PRO | PRO | SER | LYS | ||||
| 25 | LEU | PHE | ARG | PRO | GLY | ASP | GLU | PHE | VAL | ASP | ||||
| 26 | GLU | ALA | LYS | GLN | PRO | GLU | THR | LYS | ASP | GLY | ||||
| 27 | SER | ASP | SER | SER | VAL | ALA | THR | ASN | SER | THR | ||||
| 28 | PHE | THR | PRO | LEU | LEU | THR | GLN | LEU | LEU | ASN | ||||
| 29 | GLY | SER | THR | PRO | ILE | SER | SER | SER | GLU | LYS | ||||
| 30 | GLU | GLN | SER | VAL | VAL | GLY | SER | CYS | ASP | SER | ||||
| 31 | THR | GLU | SER | SER | GLN | PRO | SER | GLY | LEU | LEU | ||||
| 32 | GLU | ILE | LEU | THR | GLY | LYS | LEU | LYS | ASP | PRO | ||||
| 33 | GLU | THR | ASN | LEU | ASP | ALA | SER | GLU | GLY | THR | ||||
| 34 | PRO | SER | VAL | ASP | VAL | GLY | ALA | VAL | GLY | THR | ||||
| 35 | THR | SER | GLN | THR | ASP | ALA | SER | GLU | VAL | PHE | ||||
| 36 | GLY | ALA | VAL | LYS | SER | GLU | ALA | THR | GLN | SER | ||||
| 37 | THR | GLN | TYR | HIS | VAL | GLU | ILE | THR | GLU | HIS | ||||
| 38 | ALA | ASN | LYS | LYS | ASP | VAL | SER | THR | ALA | SER | ||||
| 39 | ASP | LYS | LYS | THR | ASP | GLU | LYS | ILE | GLN | GLN | ||||
| 40 | GLU | PRO | ALA | GLU | VAL | LYS | ARG | THR | GLN | GLU | ||||
| 41 | GLY | GLU | LEU | GLU | SER | SER | GLY | VAL | LYS | CYS | ||||
| 42 | GLN | GLN | PRO | SER | ASP | ASP | PRO | ASP | SER | LEU | ||||
| 43 | PHE | SER | PHE |
Entity 2, Calsensin 83 residues - Formula weight is not available
| 1 | MET | ALA | CYS | LYS | VAL | LYS | ALA | GLU | LEU | GLU | ||||
| 2 | ALA | ALA | PHE | LYS | LYS | LEU | ASP | ALA | ASN | GLY | ||||
| 3 | ASP | GLY | TYR | VAL | THR | ALA | LEU | GLU | LEU | GLN | ||||
| 4 | THR | PHE | MET | VAL | THR | LEU | ASP | ALA | TYR | LYS | ||||
| 5 | ALA | LEU | SER | LYS | ASP | LYS | VAL | LYS | GLU | ALA | ||||
| 6 | SER | ALA | LYS | LEU | ILE | LYS | MET | ALA | ASP | LYS | ||||
| 7 | ASN | SER | ASP | GLY | LYS | ILE | SER | LYS | GLU | GLU | ||||
| 8 | PHE | LEU | ASN | ALA | ASN | ALA | GLU | LEU | LEU | CYS | ||||
| 9 | GLN | LEU | LYS |
Samples:
sample_1: Calsensin, [U-100% 15N], 0.8 mM; sodium phosphate 50 mM; sodium chloride 75 mM; DTT 2 mM; EDTA 8 mM; sodium azide 0.02%; PP4R20 – 0.9 mM; H2O 90%; D2O 10%
sample_conditions_1: pH: 6.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMRView, Johnson, One Moon Scientific - assignments
NMR spectrometers:
- Bruker DRX 500 MHz