BMRB Entry 16762

Title:
Mapping the Ca2+-dependent binding of an invertebrate homolog of protein phosphatase 4 regulatory subunit 2 of the small EF-hand protein, calsensin
Deposition date:
2010-03-03
Original release date:
2010-03-24
Authors:
Venkitaramani, Deepa; Fulton, D.; Andreotti, Amy; Johansen, Kristen; Johansen, Jorgen
Citation:

Citation: Venkitaramani, Deepa; Fulton, D.; Andreotti, Amy; Johansen, Kristen; Johansen, Jorgen. "Mapping the Ca2+-dependent binding of an invertebrate homolog of protein phosphatase 4 regulatory subunit 2 of the small EF-hand protein, calsensin"  Biochim. Biophys. Acta 1763, 322-329 (2006).
PubMed: 16600403

Assembly members:

Assembly members:
PP4R2, polymer, 423 residues, Formula weight is not available
Calsensin, polymer, 83 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: segemented worms   Taxonomy ID: 38567   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Hirudo marmorata

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX4T3

Data sets:
  • binding_constants
Data typeCount
binding constants1

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PP4R21
2Calsensin2

Entities:

Entity 1, PP4R2 423 residues - Formula weight is not available

1   METGLYSERARGLYSGLUILEARGASPALA
2   LEUGLUGLUPHELEULYSGLNPROGLUASP
3   GLYGLUVALASNGLYILELEUGLUARGTYR
4   LEUSERARGVALALAVALTHRGLYILETHR
5   LEUTYRPROTRPSERLYSLEULYSPROLEU
6   ILETHRARGLYSMETASNLEUVALVALGLU
7   GLUPHEMETLYSGLUCYSPROMETGLUSER
8   LEUALALEUASNLYSASNVALGLUASNTHR
9   SERVALGLUASPMETLYSLYSARGLEUASN
10   ASPSERILEGLULEUPHEASNGLYILEPRO
11   PHETHRILEGLNARGLEUCYSGLULEULEU
12   THRASPGLNASNILESERLYSGLNTYRARG
13   ARGVALASPLYSPHEMETARGGLYILEGLU
14   LYSASNLEUASNVALVALTHRTHRVALASP
15   PROPHEGLYASNARGILEVALGLYASPPRO
16   LYSSERALASERASNGLYVALLEUSERALA
17   GLUASPASPCYSLEULEUTHRPROTHRSER
18   CYSTHRTHRVALLYSTHRPROPROSERTRP
19   PHESERSERLEUSERSERVALVALASNSER
20   PROASPSERTYRPROASNTRPLYSGLNSER
21   GLNASPGLUGLNGLNGLNGLNGLUGLNARG
22   ASPALAALAILESERSERSERARGGLUARG
23   LYSLYSPROARGSERGLUASPASPALAVAL
24   ASPLYSASPCYSLEUPROPROPROSERLYS
25   LEUPHEARGPROGLYASPGLUPHEVALASP
26   GLUALALYSGLNPROGLUTHRLYSASPGLY
27   SERASPSERSERVALALATHRASNSERTHR
28   PHETHRPROLEULEUTHRGLNLEULEUASN
29   GLYSERTHRPROILESERSERSERGLULYS
30   GLUGLNSERVALVALGLYSERCYSASPSER
31   THRGLUSERSERGLNPROSERGLYLEULEU
32   GLUILELEUTHRGLYLYSLEULYSASPPRO
33   GLUTHRASNLEUASPALASERGLUGLYTHR
34   PROSERVALASPVALGLYALAVALGLYTHR
35   THRSERGLNTHRASPALASERGLUVALPHE
36   GLYALAVALLYSSERGLUALATHRGLNSER
37   THRGLNTYRHISVALGLUILETHRGLUHIS
38   ALAASNLYSLYSASPVALSERTHRALASER
39   ASPLYSLYSTHRASPGLULYSILEGLNGLN
40   GLUPROALAGLUVALLYSARGTHRGLNGLU
41   GLYGLULEUGLUSERSERGLYVALLYSCYS
42   GLNGLNPROSERASPASPPROASPSERLEU
43   PHESERPHE

Entity 2, Calsensin 83 residues - Formula weight is not available

1   METALACYSLYSVALLYSALAGLULEUGLU
2   ALAALAPHELYSLYSLEUASPALAASNGLY
3   ASPGLYTYRVALTHRALALEUGLULEUGLN
4   THRPHEMETVALTHRLEUASPALATYRLYS
5   ALALEUSERLYSASPLYSVALLYSGLUALA
6   SERALALYSLEUILELYSMETALAASPLYS
7   ASNSERASPGLYLYSILESERLYSGLUGLU
8   PHELEUASNALAASNALAGLULEULEUCYS
9   GLNLEULYS

Samples:

sample_1: Calsensin, [U-100% 15N], 0.8 mM; sodium phosphate 50 mM; sodium chloride 75 mM; DTT 2 mM; EDTA 8 mM; sodium azide 0.02%; PP4R20 – 0.9 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - assignments

NMR spectrometers:

  • Bruker DRX 500 MHz

Related Database Links:

GB ABC88653 AAC46630
BMRB 6519
PDB
SP Q25088
AlphaFold Q25088