BMRB Entry 16750

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16750

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Title:
backbone chemical shifts assignments of acid-denatured c-src SH3 with increasing urea concentration
Deposition date:
2010-03-03
Original release date:
2010-05-03
Authors:
Poulsen, Flemming; Roesner, Heike
Citation:

Citation: Rosner, Heike; Poulsen, Flemming. "Residue-specific description of non-native transient structures in the ensemble of acid-denatured structures of the all-beta protein c-src SH3."  Biochemistry 49, 3246-3253 (2010).
PubMed: 20218679

Assembly members:

Assembly members:
c-src_SH3, polymer, 80 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: chicken   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gallus gallus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET_csrcSH3

Entity Sequences (FASTA):

Entity Sequences (FASTA):
c-src_SH3: MGSSHHHHHHSSGLVPRGSH MVTTFVALYDYESRTETDLS FKKGERLQIVNNTEGDWWLA HSLTTGQTGYIPSNYVAPSN

Data typeCount
13C chemical shifts762
15N chemical shifts372
1H chemical shifts372

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1c-src_SH31

Entities:

Entity 1, c-src_SH3 80 residues - Formula weight is not available

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYLEUVALPROARGGLYSERHIS
3   METVALTHRTHRPHEVALALALEUTYRASP
4   TYRGLUSERARGTHRGLUTHRASPLEUSER
5   PHELYSLYSGLYGLUARGLEUGLNILEVAL
6   ASNASNTHRGLUGLYASPTRPTRPLEUALA
7   HISSERLEUTHRTHRGLYGLNTHRGLYTYR
8   ILEPROSERASNTYRVALALAPROSERASN

Samples:

sample_2: c-src SH3, [U-13C; U-15N], 0.2 mM; sodium phosphate 250 mM; urea 3 M; DSS 0.25 mM; H2O 90%; D2O 10%

sample_1: c-src SH3, [U-13C; U-15N], 0.2 mM; sodium phosphate 250 mM; urea 2 M; DSS 0.25 mM; H2O 90%; D2O 10%

sample_3: c-src SH3, [U-13C; U-15N], 0.2 mM; sodium phosphate 250 mM; urea 4 M; DSS 0.25 mM; H2O 90%; D2O 10%

sample_4: c-src SH3, [U-13C; U-15N], 0.2 mM; sodium phosphate 250 mM; urea 6 M; DSS 0.25 mM; H2O 90%; D2O 10%

sample_5: c-src SH3, [U-13C; U-15N], 0.2 mM; sodium phosphate 250 mM; urea 8 M; DSS 0.25 mM; H2O 90%; D2O 10%

sample_6: c-src SH3, [U-13C; U-15N], 0.2 mM; sodium phosphate 250 mM; urea 9.5 M; DSS 0.25 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.25 M; pH: 2.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
3D HNCAsample_2isotropicsample_conditions_1
2D 1H-15N HSQCsample_3isotropicsample_conditions_1
3D HNCOsample_3isotropicsample_conditions_1
3D HNCAsample_3isotropicsample_conditions_1
2D 1H-15N HSQCsample_4isotropicsample_conditions_1
3D HNCOsample_4isotropicsample_conditions_1
3D HNCAsample_4isotropicsample_conditions_1
2D 1H-15N HSQCsample_5isotropicsample_conditions_1
3D HNCOsample_5isotropicsample_conditions_1
3D HNCAsample_5isotropicsample_conditions_1
2D 1H-15N HSQCsample_6isotropicsample_conditions_1
3D HNCOsample_6isotropicsample_conditions_1
3D HNCAsample_6isotropicsample_conditions_1

Software:

Analysis, CCPN - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 800 MHz

Related Database Links:

BMRB 3433
PDB
EMBL CAA23696 CAA36156
GB AAA42565 AAA42583 AAA49078 AAC37877 AAD24180
REF NP_990788 XP_001372060 XP_002191434 XP_003212004 XP_005057157
SP P00523 P00524 P00525 P14084 P14085
AlphaFold P00523 P00524 P00525 P14084 P14085

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks