BMRB Entry 16745

Title:
1H and 13C Chemical Shift Assignments for Antimicrobial Peptide Sm-AMP-1.1a
Deposition date:
2010-02-26
Original release date:
2012-08-03
Authors:
Bozin, Timur; Bocharov, Eduard; Sobol, Vladimir; Vassilevski, Alexander; Arseniev, Alexander
Citation:

Citation: Vassilevski, Alexander; Musolyamov, Alexander; Nikonorova, Alexandra; Finkina, Ekaterina; Slavokhotova, Anna; Shagin, Dmitry; Bozin, Timur; Bocharov, Eduard; Ovchinnikova, Tatyana; Arseniev, Alexander; Babakov, Alexei; Lukyanov, Sergey; Grishin, Eugene; Egorov, Tsezi. "Common chickweed (Stellaria media) antifungal peptides with chitin-binding domain provide unique plant defense strategy"  J. Biol. Chem. ., .-..

Assembly members:

Assembly members:
Sm-AMP-1.1a, polymer, 35 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Stellaria media   Taxonomy ID: 13274   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Stellaria media

Experimental source:

Experimental source:   Production method: purified from the natural source   Host organism: Stellaria media

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Sm-AMP-1.1a: SGPNGQCGPGWGGCRGGLCC SQYGYCGSGPKYCAH

Data sets:
Data typeCount
13C chemical shifts90
1H chemical shifts190
coupling constants60

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Sm-AMP-1.1a monomer1

Entities:

Entity 1, Sm-AMP-1.1a monomer 35 residues - Formula weight is not available

1   SERGLYPROASNGLYGLNCYSGLYPROGLY
2   TRPGLYGLYCYSARGGLYGLYLEUCYSCYS
3   SERGLNTYRGLYTYRCYSGLYSERGLYPRO
4   LYSTYRCYSALAHIS

Samples:

sample_1: Sm-AMP-1.1a 0.5 mM; H2O 90%; D2O 10%; NaCl 10 mM

sample_2: Sm-AMP-1.1a 0.5 mM; D2O 100%; NaCl 10 mM

sample_conditions_1: ionic strength: 20 mM; pH: 4.5; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_2isotropicsample_conditions_1
2D 1H-1H TOCSYsample_2isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_1

Software:

TOPSPIN v2.1, Bruker Biospin - collection

XEASY, Bartels et al. - chemical shift assignment, data analysis

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

NMR spectrometers:

  • Bruker Avance 700 MHz

Related Database Links:

PDB