BMRB Entry 16721

Title:
Solution structure of BRD1 PHD1 finger
Deposition date:
2010-02-12
Original release date:
2012-08-03
Authors:
Zhang, Jiahai; Wu, Jihui; Shi, Yunyu
Citation:

Citation: Qin, Su; Zhang, Jiahai; Wu, Jihui; Shi, Yunyu. "Solution structure of BRD1 PHD1 finger"  .

Assembly members:

Assembly members:
BRD1 PHD1 finger, polymer, 71 residues, 6966.939 Da.
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: p28

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts223
15N chemical shifts65
1H chemical shifts385

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1BRD1 PHD1 finger1
2ZINC ION_12
3ZINC ION_22

Entities:

Entity 1, BRD1 PHD1 finger 71 residues - 6966.939 Da.

1   METGLYHISHISHISHISHISHISMETGLN
2   SERLEUILEASPGLUASPALAVALCYSSER
3   ILECYSMETASPGLYGLUSERGLNASNSER
4   ASNVALILELEUPHECYSASPMETCYSASN
5   LEUALAVALHISGLNGLUCYSTYRGLYVAL
6   PROTYRILEPROGLUGLYGLNTRPLEUCYS
7   ARGHISCYSLEUGLNSERARGALAARGPRO
8   ALA

Entity 2, ZINC ION_1 - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: ZINC ION 2 mM; entity_1, [U-100% 13C; U-100% 15N], 1 mM; sodium chloride 150 mM; Bis-Tris 20 mM; H2O 90%; D2O 10%

sample_2: ZINC ION 2 mM; entity_1, [U-100% 13C; U-100% 15N], 1 mM; Bis-Tris 20 mM; sodium chloride 150 mM; D2O 100%

sample_conditions_1: ionic strength: 0.15 M; pH: 6.7; pressure: 1 atm; temperature: 293 K

sample_conditions_2: ionic strength: 0.15 M; pD: 6.7; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_2isotropicsample_conditions_2

Software:

NMRPipe v2.2, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY v3, Goddard - chemical shift assignment, data analysis

CNS v1.2, Brunger, Adams, Clore, Gros, Nilges and Read - refinement, structure solution

TALOS, Cornilescu, Delaglio and Bax - processing

Molmol v2K.2, Koradi, Billeter and Wuthrich - data analysis

NMR spectrometers:

  • Bruker DMX 500 MHz

Related Database Links:

BMRB 17244
PDB
REF XP_009232775 XP_009232776 XP_009232777 XP_009232778 XP_009232779

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks