BMRB Entry 16711

Name: NMR structure of the protein NP_415897.1
Published: 2012-08-03

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PDB ID: 2kts
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16711
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR structure of the protein NP_415897.1

Deposition date:

2010-02-06

Original release date:

2012-08-03

Authors:

Serrano, Pedro; Jaudzems, Kristaps; Geralt, Michael; Horst, Reto; Wuthrich, Kurt; Wilson, Ian

Citation:

Citation: Serrano, Pedro; Jaudzems, Kristaps; Geralt, Michael; Horst, Reto; Wilson, Ian; Wuthrich, Kurt. "NMR structure of the protein NP_415897.1" .

Assembly members:

Assembly members:
NP_415897.1, polymer, 117 residues, 12846.825 Da.

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET25b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
NP_415897.1: MVTPEQLQHHRFVLESVNGK PVTSDKNPPEISFGEKMMIS GSMCNRFSGEGKLSNGELTA KGLAMTRMMCANPQLNELDN TISEMLKEGAQVDLTANQLT LATAKQTLTYKLADLMN

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	387
15N chemical shifts	131
1H chemical shifts	822

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	NP_415897.1	1

Entities:

Entity 1, NP_415897.1 117 residues - 12846.825 Da.

1

MET

VAL

THR

PRO

GLU

GLN

LEU

GLN

HIS

2

ARG

PHE

VAL

LEU

GLU

SER

VAL

ASN

GLY

LYS

3

PRO

VAL

THR

SER

ASP

LYS

ASN

PRO

GLU

4

ILE

SER

PHE

GLY

GLU

LYS

MET

ILE

SER

5

GLY

SER

MET

CYS

ASN

ARG

PHE

SER

GLY

GLU

6

GLY

LYS

LEU

SER

ASN

GLY

GLU

LEU

THR

ALA

7

LYS

GLY

LEU

ALA

MET

THR

ARG

MET

CYS

8

ALA

ASN

PRO

GLN

LEU

ASN

GLU

LEU

ASP

ASN

9

THR

ILE

SER

GLU

MET

LEU

LYS

GLU

GLY

ALA

10

GLN

VAL

ASP

LEU

THR

ALA

ASN

GLN

LEU

THR

11

LEU

ALA

THR

ALA

LYS

GLN

THR

LEU

THR

TYR

12

LYS

LEU

ALA

ASP

LEU

MET

ASN

Samples:

sample_1: NP_415897.1, [U-98% 13C; U-98% 15N], 1.2 mM; sodium phosphate 20 mM; sodium chloride 50 mM; sodium azide 0.05%; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 0.1 M; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY (Ali)	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY (Aro)	sample_1	isotropic	sample_conditions_1
APSY 5D-CBCACONH	sample_1	isotropic	sample_conditions_1
APSY 5D-HACACONH	sample_1	isotropic	sample_conditions_1
APSY 4D-HACANH	sample_1	isotropic	sample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

CARA, Keller and Wuthrich - chemical shift assignment

TOPSPIN, Bruker Biospin - collection, processing

OPAL, Luginbuhl, Guntert, Billeter and Wuthrich - refinement

UNIO, Herrmann and Wuthrich - chemical shift assignment, peak picking, structure solution

NMR spectrometers:

Bruker Avance 600 MHz
Bruker Avance 800 MHz

PDB	2KTS
DBJ	BAA14984 BAB35424 BAG76988 BAI25261 BAI30341
EMBL	CAP75894 CAQ31880 CAQ98238 CAR02814 CAR07740
GB	AAC74461 AAG56381 AAN80290 AAZ88432 ABB66292
REF	NP_310028 NP_415897 WP_000129114 WP_001295716 WP_001296048
SP	P52644
AlphaFold	P52644