BMRB Entry 16668

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR16668

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Title:
GTPase RhoA-GDP
Deposition date:
2010-01-07
Original release date:
2010-02-16
Authors:
Gasmi-Seabrook, Genevieve; Ikura, Mitsu
Citation:

Citation: Gasmi-Seabrook, Genevieve; Marshall, Christopher; Cheung, Melissa; Kim, Bryan; Wang, Feng; Jang, Ying Ju; Mak, Tak; Stambolic, Vuk; Ikura, Mitsuhiko. "Real-time NMR study of guanine nucleotide exchange and activation of RhoA by PDZ-RhoGEF."  J. Biol. Chem. 285, 5137-5145 (2010).
PubMed: 20018869

Assembly members:

Assembly members:
GTPase_RhoA, polymer, 181 residues, 443.201 Da.
GDP, polymer, . residues, 443.201 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: purified from the natural source   Host organism: Escherichia coli   Vector: pET28A

Entity Sequences (FASTA):

Entity Sequences (FASTA):
GTPase_RhoA: MAAIRKKLVIVGDGACGKTC LLIVFSKDQFPEVYVPTVFE NYVADIEVDGKQVELALWDT AGQEDYDRLRPLSYPDTDVI LMCFSIDSPDSLENIPEKWT PEVKHFCPNVPIILVGNKKD LRNDEHTRRELAKMKQEPVK PEEGRDMANRIGAFGYMECS AKTKDGVREVFEMATRAALQ A

Data sets:
Data typeCount
13C chemical shifts479
15N chemical shifts152
1H chemical shifts270

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1protein1
2nucleotide2

Entities:

Entity 1, protein 181 residues - 443.201 Da.

1   METALAALAILEARGLYSLYSLEUVALILE
2   VALGLYASPGLYALACYSGLYLYSTHRCYS
3   LEULEUILEVALPHESERLYSASPGLNPHE
4   PROGLUVALTYRVALPROTHRVALPHEGLU
5   ASNTYRVALALAASPILEGLUVALASPGLY
6   LYSGLNVALGLULEUALALEUTRPASPTHR
7   ALAGLYGLNGLUASPTYRASPARGLEUARG
8   PROLEUSERTYRPROASPTHRASPVALILE
9   LEUMETCYSPHESERILEASPSERPROASP
10   SERLEUGLUASNILEPROGLULYSTRPTHR
11   PROGLUVALLYSHISPHECYSPROASNVAL
12   PROILEILELEUVALGLYASNLYSLYSASP
13   LEUARGASNASPGLUHISTHRARGARGGLU
14   LEUALALYSMETLYSGLNGLUPROVALLYS
15   PROGLUGLUGLYARGASPMETALAASNARG
16   ILEGLYALAPHEGLYTYRMETGLUCYSSER
17   ALALYSTHRLYSASPGLYVALARGGLUVAL
18   PHEGLUMETALATHRARGALAALALEUGLN
19   ALA

Entity 2, nucleotide - 443.201 Da.

1   GDP

Related Database Links:

BMRB 16669
PDB 1A2B 1CC0 1CXZ 1DPF 1FTN 1KMQ 1LB1 1OW3 1S1C 1TX4 1X86 1XCG 2RGN 3KZ1 3LW8 3LWN 3LXR 3MSX 3T06 3TVD 4D0N 4F38 4XH9 4XOI 4XSG 4XSH 5A0F 5BWM
DBJ BAA20863 BAC36896 BAC38971 BAC99017 BAD96276
EMBL CAA28690 CAA39802 CAC08447 CAC28868 CAE46190
GB AAA30409 AAA50612 AAC18962 AAC23710 AAC33178
REF NP_001003273 NP_001025020 NP_001038815 NP_001079729 NP_001080441
SP P24406 P61585 P61586 P61589 Q5REY6
TPG DAA16889
AlphaFold P24406 P61585 P61586 P61589 Q5REY6

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks