BMRB Entry 16668

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR16668

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Title:
GTPase RhoA-GDP
Deposition date:
2010-01-07
Original release date:
2010-02-16
Authors:
Gasmi-Seabrook, Genevieve; Ikura, Mitsu
Citation:

Citation: Gasmi-Seabrook, Genevieve; Marshall, Christopher; Cheung, Melissa; Kim, Bryan; Wang, Feng; Jang, Ying Ju; Mak, Tak; Stambolic, Vuk; Ikura, Mitsuhiko. "Real-time NMR study of guanine nucleotide exchange and activation of RhoA by PDZ-RhoGEF."  J. Biol. Chem. 285, 5137-5145 (2010).
PubMed: 20018869

Assembly members:

Assembly members:
GTPase_RhoA, polymer, 181 residues, 443.201 Da.
GDP, polymer, . residues, 443.201 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: purified from the natural source   Host organism: Escherichia coli   Vector: pET28A

Entity Sequences (FASTA):

Entity Sequences (FASTA):
GTPase_RhoA: MAAIRKKLVIVGDGACGKTC LLIVFSKDQFPEVYVPTVFE NYVADIEVDGKQVELALWDT AGQEDYDRLRPLSYPDTDVI LMCFSIDSPDSLENIPEKWT PEVKHFCPNVPIILVGNKKD LRNDEHTRRELAKMKQEPVK PEEGRDMANRIGAFGYMECS AKTKDGVREVFEMATRAALQ A

Data sets:
Data typeCount
13C chemical shifts479
15N chemical shifts152
1H chemical shifts270

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1protein1
2nucleotide2

Entities:

Entity 1, protein 181 residues - 443.201 Da.

1   METALAALAILEARGLYSLYSLEUVALILE
2   VALGLYASPGLYALACYSGLYLYSTHRCYS
3   LEULEUILEVALPHESERLYSASPGLNPHE
4   PROGLUVALTYRVALPROTHRVALPHEGLU
5   ASNTYRVALALAASPILEGLUVALASPGLY
6   LYSGLNVALGLULEUALALEUTRPASPTHR
7   ALAGLYGLNGLUASPTYRASPARGLEUARG
8   PROLEUSERTYRPROASPTHRASPVALILE
9   LEUMETCYSPHESERILEASPSERPROASP
10   SERLEUGLUASNILEPROGLULYSTRPTHR
11   PROGLUVALLYSHISPHECYSPROASNVAL
12   PROILEILELEUVALGLYASNLYSLYSASP
13   LEUARGASNASPGLUHISTHRARGARGGLU
14   LEUALALYSMETLYSGLNGLUPROVALLYS
15   PROGLUGLUGLYARGASPMETALAASNARG
16   ILEGLYALAPHEGLYTYRMETGLUCYSSER
17   ALALYSTHRLYSASPGLYVALARGGLUVAL
18   PHEGLUMETALATHRARGALAALALEUGLN
19   ALA

Entity 2, nucleotide - 443.201 Da.

1   GDP

Samples:

sample_1: GTPase RhoA, [U-100% 13C; U-100% 15N], 0.3 mM; D2O 10%; H2O 90%

sample_conditions_1: ionic strength: 100 mM; pH: 7.0; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1anisotropicsample_conditions_1
3D CBCA(CO)NHsample_1anisotropicsample_conditions_1
3D HNCACBsample_1anisotropicsample_conditions_1
3D HNCOsample_1anisotropicsample_conditions_1
3D HN(CA)COsample_1anisotropicsample_conditions_1
3D HNHAsample_1anisotropicsample_conditions_1
3D 1H-15N NOESYsample_1anisotropicsample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Bruker Avance 800 MHz

Related Database Links:

BMRB 16669
PDB
DBJ BAA20863 BAC36896 BAC38971 BAC99017 BAD96276
EMBL CAA28690 CAA39802 CAC08447 CAC28868 CAE46190
GB AAA30409 AAA50612 AAC18962 AAC23710 AAC33178
REF NP_001003273 NP_001025020 NP_001038815 NP_001079729 NP_001080441
SP P24406 P61585 P61586 P61589 Q5REY6
TPG DAA16889
AlphaFold P24406 P61585 P61586 P61589 Q5REY6

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks