BMRB Entry 16652

Title:
Solution NMR Structure of asl3597 from Nostoc sp. PCC7120. Northeast Structural Genomics Consortium Target ID Nsr244.
Deposition date:
2009-12-22
Original release date:
2011-11-09
Authors:
Feldmann, Erik; Ramelot, Theresa; Yang, Yunhuang; Lee, Dan; Ciccosanti, Colleen; Janjua, Haleema; Liu, J.; Rost, Burkhard; Acton, Thomas; Xiao, R.; Everett, John; Montelione, Gaetano; Kennedy, Michael
Citation:

Citation: Feldmann, Erik; Ramelot, Theresa; Yang, Yunhuang; Lee, Dan; Ciccosanti, Colleen; Janjua, Haleema; Liu, J.; Rost, Burkhard; Swapna, Thomas; Acton, Thomas; Xiao, R.; Everett, John; Montelione, Gaetano; Kennedy, Michael. "Solution NMR structure of asl3597 from Nostoc sp. PCC7120. Northeast Structural Genomics Consortium target NsR244."  .

Assembly members:

Assembly members:
asl3597, polymer, 94 residues, 10974 Da.

Natural source:

Natural source:   Common Name: Nostoc sp. PCC7120   Taxonomy ID: 103690   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Nostoc Nostoc sp. PCC7120

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Nostoc sp. PCC7120   Vector: pET21-23C

Data typeCount
13C chemical shifts370
15N chemical shifts79
1H chemical shifts594

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1asl35971

Entities:

Entity 1, asl3597 94 residues - 10974 Da.

full length followed by 8 non-native C-terminal residues (LEHHHHHH)

1   METPROASPPROLEUMETTYRGLNGLNASP
2   ASNPHEVALVALLEUGLUTHRASNGLNPRO
3   GLUGLNPHELEUTHRTHRILEGLULEULEU
4   GLULYSLEULYSGLYGLULEUGLULYSILE
5   SERPHESERASPLEUPROLEUGLULEUGLN
6   LYSLEUASPSERLEUPROALAGLNALAGLN
7   HISLEUILEASPTHRSERCYSGLULEUASP
8   VALGLYALAGLYLYSTYRLEUGLNTRPTYR
9   ALAVALARGLEUGLULYSLEUGLUHISHIS
10   HISHISHISHIS

Samples:

NC_sample: entity, [U-100% 13C; U-100% 15N], 1.1 ± 0.1 mM; MES 20 ± 0.1 mM; sodium chloride 200 ± 5 mM; DTT 10 ± 0.5 mM; calcium chloride 5 ± 0.2 mM; sodium azide 0.02 ± 0.001 %

NC5_sample: entity, [U-5% 13C; U-100% 15N], 1.1 ± 0.1 mM; MES 20 ± 0.1 mM; sodium chloride 200 ± 5 mM; DTT 10 ± 0.5 mM; calcium chloride 5 ± 0.2 mM; sodium azide 0.02 ± 0.001 %

NC_sample_in_D2O: entity, [U-100% 13C; U-100% 15N], 1.0 ± 0.1 mM; MES 20 ± 0.1 mM; sodium chloride 200 ± 5 mM; DTT 10 ± 0.5 mM; calcium chloride 5 ± 0.2 mM; sodium azide 0.02 ± 0.001 %

sample_conditions_1: ionic strength: 0.2 M; pH: 6.5; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCNC_sampleisotropicsample_conditions_1
2D 1H-13C HSQCNC_sampleisotropicsample_conditions_1
2D 1H-13C HSQCNC5_sampleisotropicsample_conditions_1
2D 1H-15N HSQCNC_sampleisotropicsample_conditions_1
3D 1H-15N NOESYNC_sampleisotropicsample_conditions_1
3D 1H-13C NOESYNC_sampleisotropicsample_conditions_1
4D CC NOESYNC_sample_in_D2Oisotropicsample_conditions_1
3D HNCONC_sampleisotropicsample_conditions_1
3D HNCANC_sampleisotropicsample_conditions_1
3D HNCACBNC_sampleisotropicsample_conditions_1
3D CBCA(CO)NHNC_sampleisotropicsample_conditions_1
3D HBHA(CO)NHNC_sampleisotropicsample_conditions_1
3D H(C)CH-TOCSYNC_sampleisotropicsample_conditions_1
3D (H)CCH-TOCSYNC_sampleisotropicsample_conditions_1
3D HN(CO)CANC_sampleisotropicsample_conditions_1
3D H(C)CH COSYNC_sampleisotropicsample_conditions_1

Software:

NMRPipe v2008, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

VNMR v6.1C, Varian - collection

TOPSPIN v2.1.3, Bruker Biospin - collection

AutoStruct v2.2.1, Huang, Tejero, Powers and Montelione - data analysis

X-PLOR NIH v2.20, Schwieters, Kuszewski, Tjandra and Clore - structure solution

SPARKY v3.113, Goddard - data analysis

PSVS v1.4, Bhattacharya and Montelione - structure solution

AutoAssign v2.3.0, Zimmerman, Moseley, Kulikowski and Montelione - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Bruker AvanceIII 850 MHz

Related Database Links:

PDB
DBJ BAB75296
REF WP_010997746

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks