BMRB Entry 16623

Title:
The U-box domain of mus musculus E4B
Deposition date:
2009-12-03
Original release date:
2010-01-28
Authors:
Nordquist, Kyle; Soss, Sarah; Chazin, Walter
Citation:

Citation: Nordquist, Kyle; Dimitrova, Yoana; Brzovic, Peter; Ridenour, Whitney; Munro, Kim; Soss, Sarah; Caprioli, Richard; Klevit, Rachel; Chazin, Walter. "Structural and functional characterization of the monomeric U-box domain from E4B."  Biochemistry 49, 347-355 (2010).
PubMed: 20017557

Assembly members:

Assembly members:
E4B, polymer, 85 residues, 9779.069 Da.

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pBG102

Data sets:
Data typeCount
13C chemical shifts210
15N chemical shifts76
1H chemical shifts555

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1E4B1

Entities:

Entity 1, E4B 85 residues - 9779.069 Da.

1   PROGLYSERALAGLUILEASPTYRSERASP
2   ALAPROASPGLUPHEARGASPPROLEUMET
3   ASPTHRLEUMETTHRASPPROVALARGLEU
4   PROSERGLYTHRVALMETASPARGSERILE
5   ILELEUARGHISLEULEUASNSERPROTHR
6   ASPPROPHEASNARGGLNMETLEUTHRGLU
7   SERMETLEUGLUPROVALPROGLULEULYS
8   GLUGLNILEGLNALATRPMETARGGLULYS
9   GLNSERSERASPHIS

Samples:

sample_1: U-box domain of E4B, [U-100% 13C; U-100% 15N], 750 uM; H2O 90%; D2O 10%

sample_2: U-box domain of E4B 1 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D HCCH-COSYsample_1isotropicsample_conditions_1

Software:

NMRPipe v2009.015.15.35, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY v3.113, Goddard - chemical shift assignment, peak picking

CYANA v2..1, Guntert, Mumenthaler and Wuthrich - structure solution

AMBER v9, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollm - refinement

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz

Related Database Links:

PDB
DBJ BAC26672 BAC56586 BAC65627
GB AAG17287 AAH67402 AAH75620
REF NP_001258127 NP_071305 XP_005353748 XP_005353749 XP_006239449
SP Q9ES00
AlphaFold Q9ES00

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks