BMRB Entry 16619

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16619

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Title:
Sequence-specific 1H, 13C, and 15N resonance assignments of Diva (Boo), an apoptosis regulator of the Bcl-2 family
Deposition date:
2009-11-27
Original release date:
2010-02-08
Authors:
Sborgi, Lorenzo; de Alba, Eva
Citation:

Citation: Sborgi, Lorenzo; de Alba, Eva. "Sequence-specific 1H, 13C, and 15N resonance assignments of Diva (Boo), an apoptosis regulator of the Bcl-2 family."  Biomol. NMR Assignments 4, 65-68 (2010).
PubMed: 20127209

Assembly members:

Assembly members:
Diva_(Boo), polymer, 160 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pBAT4

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Diva_(Boo): MADSQDPLHERTRRLLSDYI FFCAREPDTPEPPPTSVEAA LLRSVTRQIQQEHQEFFSSF CESRGNRLELVKQMADKLLS KDQDFSWSQLVMLLAFAGTL MNQGPYMAVKQKRDLGNRVI VTRDCCLIVNFLYNLLMGRR HRARLEALGGWDGFCRFFKN

Data sets:
Data typeCount
13C chemical shifts699
15N chemical shifts162
1H chemical shifts1096

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Diva (Boo)1

Entities:

Entity 1, Diva (Boo) 160 residues - Formula weight is not available

1   METALAASPSERGLNASPPROLEUHISGLU
2   ARGTHRARGARGLEULEUSERASPTYRILE
3   PHEPHECYSALAARGGLUPROASPTHRPRO
4   GLUPROPROPROTHRSERVALGLUALAALA
5   LEULEUARGSERVALTHRARGGLNILEGLN
6   GLNGLUHISGLNGLUPHEPHESERSERPHE
7   CYSGLUSERARGGLYASNARGLEUGLULEU
8   VALLYSGLNMETALAASPLYSLEULEUSER
9   LYSASPGLNASPPHESERTRPSERGLNLEU
10   VALMETLEULEUALAPHEALAGLYTHRLEU
11   METASNGLNGLYPROTYRMETALAVALLYS
12   GLNLYSARGASPLEUGLYASNARGVALILE
13   VALTHRARGASPCYSCYSLEUILEVALASN
14   PHELEUTYRASNLEULEUMETGLYARGARG
15   HISARGALAARGLEUGLUALALEUGLYGLY
16   TRPASPGLYPHECYSARGPHEPHELYSASN

Samples:

sample_1: Diva (Boo), [U-13C; U-15N], ~0.2 mM; sodium acetate, [U-2H], 20 mM; sodium azide 0.1 mM; TCEP, [U-2H], 5 mM

sample_2: Diva (Boo), [U-13C; U-15N], ~0.1 mM; sodium acetate, [U-2H], 20 mM; sodium azide 0.1 mM; TCEP, [U-2H], 5 mM

sample_conditions_1: pH: 5.4; pressure: 1 atm; temperature: 308 K

sample_conditions_2: pH*: 5; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_2
4D 1H-13C NOESYsample_2isotropicsample_conditions_2
3D 1H-13C NOESYsample_2isotropicsample_conditions_2

Software:

PIPP, Garrett - chemical shift assignment, peak picking

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Bruker Avance III 600 MHz
  • Bruker Avance 600 MHz

Related Database Links:

PDB
DBJ BAE22856 BAE26403 BAE36742
GB AAC83150 AAD08703 AAH52690 EDL26315
REF NP_038507
SP Q9Z0F3
AlphaFold Q9Z0F3

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks