BMRB Entry 16618

Name: NMR structure of the J-domain (residues 2-72) in the Escherichia coli CbpA
Published: 2010-05-05

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16618

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR structure of the J-domain (residues 2-72) in the Escherichia coli CbpA

Deposition date:

2009-11-25

Original release date:

2010-05-05

Authors:

Ekiel, Irena; Sarri-Sarraf, Naghmeh

Citation:

Citation: Sarraf, Naghmeh; Baardsnes, Jason; Cheng, Jing; Cygler, Maureen; Ekiel, Miroslaw. "Structural basis of the regulation of the CbpA co-chaperone by its specific modulator CbpM." J. Mol. Biol. 398, 111-121 (2010).
PubMed: 20226195

Assembly members:

Assembly members:
J-domain of CbpA, polymer, 73 residues, 8720.918 Da.

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pFO1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
J-domain of CbpA: GSELKDYYAIMGVKPTDDLK TIKTAYRRLARKYHPDVSKE PDAEARFKEVAEAWEVLSDE QRRAEYDQMWQHR

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	331
15N chemical shifts	70
1H chemical shifts	484

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	J-domain of CbpA	1

Entities:

Entity 1, J-domain of CbpA 73 residues - 8720.918 Da.

GS at the beginning of the sequence is the cloning artifact.

1

GLY

SER

GLU

LEU

LYS

ASP

TYR

ALA

ILE

2

MET

GLY

VAL

LYS

PRO

THR

ASP

LEU

LYS

3

THR

ILE

LYS

THR

ALA

TYR

ARG

LEU

ALA

4

ARG

LYS

TYR

HIS

PRO

ASP

VAL

SER

LYS

GLU

5

PRO

ASP

ALA

GLU

ALA

ARG

PHE

LYS

GLU

VAL

6

ALA

GLU

ALA

TRP

GLU

VAL

LEU

SER

ASP

GLU

7

GLN

ARG

ALA

GLU

TYR

ASP

GLN

MET

TRP

8

GLN

HIS

ARG

Samples:

sample_1: J-domain of CbpA, [U-13C; U-15N], 1 mM; sodium phosphate 50 mM; sodium chloride 250 mM; DTT 1 mM

sample_conditions_1: ionic strength: 300 mM; pH: 6.8; pressure: 1 atm; temperature: 300 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1

Software:

xwinnmr, Bruker Biospin - collection

NMR spectrometers:

Bruker Avance 500 MHz

PDB	2KQX 3UCS
DBJ	BAA03950 BAA36142 BAB34578 BAG76586 BAI24855
EMBL	CAD08237 CAP75502 CAQ31527 CAQ88675 CAQ97907
GB	AAC45599 AAC74085 AAG55547 AAL20044 AAN42630
PIR	AB0632
REF	NP_309182 NP_415520 NP_455607 NP_460085 NP_706923
SP	A1A9Q7 A7ZKA5 A7ZYV2 A8AI78 A9MH53
AlphaFold	A1A9Q7 A7ZKA5 A7ZYV2 A8AI78 A9MH53