BMRB Entry 16598

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16598

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
1H, 13C and 15N backbone resonance assignments of cTEM-17m, a TEM-1/PSE-4 class A beta-lactamases chimera
Deposition date:
2009-11-06
Original release date:
2010-05-05
Authors:
Morin, Sebastien; Clouthier, Christopher; Gobeil, Sophie; Gagne, Stephane; Pelletier, Joelle
Citation:

Citation: Morin, Sebastien; Clouthier, Christopher; Gobeil, Sophie; Pelletier, Joelle; Gagne, Stephane. "Backbone resonance assignments of an artificially engineered TEM-1/PSE-4 Class A -lactamase chimera."  Biomol. NMR Assignments 4, 127-130 (2010).
PubMed: 20383614

Assembly members:

Assembly members:
cTEM-17m, polymer, 263 residues, 28838.9 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET24

Entity Sequences (FASTA):

Entity Sequences (FASTA):
cTEM-17m: HPETLVKVKDAEDQLGARVG YIELDLNSGKILESFRPEER FPMMSTFKVLLCGAVLSRVD AGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSA AITMSDNTAANLLLTTIGGP KELTDFLRQIGDKETRLDRI EPDLNEGKLGDLRDTTTPKA IASTLRKLLTGELLTLASRQ QLIDWMEADKVAGPLLRSAL PAGWFIADKSGAGERGSRGI IAALGPDGKPSRIVVIYTTG SQATMDERNRQIAEIGASLI KHW

Data sets:
Data typeCount
13C chemical shifts680
15N chemical shifts228
1H chemical shifts228

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1cTEM-17m1

Entities:

Entity 1, cTEM-17m 263 residues - 28838.9 Da.

This is the Ambler numbering scheme used for class A beta-lactamases.

1   HISPROGLUTHRLEUVALLYSVALLYSASP
2   ALAGLUASPGLNLEUGLYALAARGVALGLY
3   TYRILEGLULEUASPLEUASNSERGLYLYS
4   ILELEUGLUSERPHEARGPROGLUGLUARG
5   PHEPROMETMETSERTHRPHELYSVALLEU
6   LEUCYSGLYALAVALLEUSERARGVALASP
7   ALAGLYGLNGLUGLNLEUGLYARGARGILE
8   HISTYRSERGLNASNASPLEUVALGLUTYR
9   SERPROVALTHRGLULYSHISLEUTHRASP
10   GLYMETTHRVALARGGLULEUCYSSERALA
11   ALAILETHRMETSERASPASNTHRALAALA
12   ASNLEULEULEUTHRTHRILEGLYGLYPRO
13   LYSGLULEUTHRASPPHELEUARGGLNILE
14   GLYASPLYSGLUTHRARGLEUASPARGILE
15   GLUPROASPLEUASNGLUGLYLYSLEUGLY
16   ASPLEUARGASPTHRTHRTHRPROLYSALA
17   ILEALASERTHRLEUARGLYSLEULEUTHR
18   GLYGLULEULEUTHRLEUALASERARGGLN
19   GLNLEUILEASPTRPMETGLUALAASPLYS
20   VALALAGLYPROLEULEUARGSERALALEU
21   PROALAGLYTRPPHEILEALAASPLYSSER
22   GLYALAGLYGLUARGGLYSERARGGLYILE
23   ILEALAALALEUGLYPROASPGLYLYSPRO
24   SERARGILEVALVALILETYRTHRTHRGLY
25   SERGLNALATHRMETASPGLUARGASNARG
26   GLNILEALAGLUILEGLYALASERLEUILE
27   LYSHISTRP

Related Database Links:

PDB 4ID4 4QY5 4QY6 4R4R 4R4S

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks