BMRB Entry 16591

Title:
1H and 15N chemical shifts for the immune inhibitory receptor LAIR-1 collagen binding domain
Deposition date:
2009-10-29
Original release date:
2010-02-01
Authors:
Brondijk, Harma; de Ruiter, Talitha; Ballering, Joost; Wienk, Hans; Lebbink, Robert Jan; van Ingen, Hugo; Boelens, Rolf; Farndale, Richard; Meyaard, Linde; Huizinga, Eric
Citation:

Citation: Brondijk, T. Harma C; de Ruiter, Talitha; Ballering, Joost; Wienk, Hans; Lebbink, Robert Jan; van Ingen, Hugo; Boelens, Rolf; Farndale, Richard; Meyaard, Linde; Huizinga, Eric. "Crystal structure and collagen-binding site of immune inhibitory receptor LAIR-1: unexpected implications for collagen binding by platelet receptor GPVI."  Blood 115, 1364-1373 (2010).
PubMed: 20007810

Assembly members:

Assembly members:
LAIR-1, polymer, 105 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET3xa

Data sets:
Data typeCount
15N chemical shifts97
1H chemical shifts567

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1LAIR-11

Entities:

Entity 1, LAIR-1 105 residues - Formula weight is not available

1   SERGLNGLUGLUASPLEUPROARGPROSER
2   ILESERALAGLUPROGLYTHRVALILEPRO
3   LEUGLYSERHISVALTHRPHEVALCYSARG
4   GLYPROVALGLYVALGLNTHRPHEARGLEU
5   GLUARGGLUSERARGSERTHRTYRASNASP
6   THRGLUASPVALSERGLNALASERPROSER
7   GLUSERGLUALAARGPHEARGILEASPSER
8   VALSERGLUGLYASNALAGLYPROTYRARG
9   CYSILETYRTYRLYSPROPROLYSTRPSER
10   GLUGLNSERASPTYRLEUGLULEULEUVAL
11   LYSGLUALAALAALA

Samples:

sample_1: LAIR-1, [U-100% 15N], 0.8 mM; potassium phosphate 10 mM; D2O 10%; H2O 90%

sample_conditions_1: ionic strength: 10 mM; pH: 7.2; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker Avance III 600 MHz

Related Database Links:

PDB
DBJ BAG53543
GB AAF17107 AAF71274 EAW72234 EAW72235 EAW72237
REF XP_011546916

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks