BMRB Entry 16591

Name: 1H and 15N chemical shifts for the immune inhibitory receptor LAIR-1 collagen binding domain
Published: 2010-02-01

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16591

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

1H and 15N chemical shifts for the immune inhibitory receptor LAIR-1 collagen binding domain

Deposition date:

2009-10-29

Original release date:

2010-02-01

Authors:

Brondijk, Harma; de Ruiter, Talitha; Ballering, Joost; Wienk, Hans; Lebbink, Robert Jan; van Ingen, Hugo; Boelens, Rolf; Farndale, Richard; Meyaard, Linde; Huizinga, Eric

Citation:

Citation: Brondijk, T. Harma C; de Ruiter, Talitha; Ballering, Joost; Wienk, Hans; Lebbink, Robert Jan; van Ingen, Hugo; Boelens, Rolf; Farndale, Richard; Meyaard, Linde; Huizinga, Eric. "Crystal structure and collagen-binding site of immune inhibitory receptor LAIR-1: unexpected implications for collagen binding by platelet receptor GPVI." Blood 115, 1364-1373 (2010).
PubMed: 20007810

Assembly members:

Assembly members:
LAIR-1, polymer, 105 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET3xa

Entity Sequences (FASTA):

Entity Sequences (FASTA):
LAIR-1: SQEEDLPRPSISAEPGTVIP LGSHVTFVCRGPVGVQTFRL ERESRSTYNDTEDVSQASPS ESEARFRIDSVSEGNAGPYR CIYYKPPKWSEQSDYLELLV KEAAA

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
15N chemical shifts	97
1H chemical shifts	567

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	LAIR-1	1

Entities:

Entity 1, LAIR-1 105 residues - Formula weight is not available

1

SER

GLN

GLU

ASP

LEU

PRO

ARG

PRO

SER

2

ILE

SER

ALA

GLU

PRO

GLY

THR

VAL

ILE

PRO

3

LEU

GLY

SER

HIS

VAL

THR

PHE

VAL

CYS

ARG

4

GLY

PRO

VAL

GLY

VAL

GLN

THR

PHE

ARG

LEU

5

GLU

ARG

GLU

SER

ARG

SER

THR

TYR

ASN

ASP

6

THR

GLU

ASP

VAL

SER

GLN

ALA

SER

PRO

SER

7

GLU

SER

GLU

ALA

ARG

PHE

ARG

ILE

ASP

SER

8

VAL

SER

GLU

GLY

ASN

ALA

GLY

PRO

TYR

ARG

9

CYS

ILE

TYR

LYS

PRO

LYS

TRP

SER

10

GLU

GLN

SER

ASP

TYR

LEU

GLU

LEU

VAL

11

LYS

GLU

ALA

Samples:

sample_1: LAIR-1, [U-100% 15N], 0.8 mM; potassium phosphate 10 mM; D2O 10%; H2O 90%

sample_conditions_1: ionic strength: 10 mM; pH: 7.2; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

Bruker Avance III 600 MHz

PDB	3KGR 3RP1
DBJ	BAG53543
GB	AAF17107 AAF71274 EAW72234 EAW72235 EAW72237
REF	XP_011546916