BMRB Entry 16567

Name: 1H, 13C, and 15N chemical shift assignments for the RNA recognition motif of Nab3
Published: 2010-05-04

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16567

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C, and 15N chemical shift assignments for the RNA recognition motif of Nab3

Deposition date:

2009-10-19

Original release date:

2010-05-04

Authors:

Stefl, Richard; Kubicek, Karel; Pergoli, Roberto; Hobor, Fruzsina; Pasulka, Josef

Citation:

Citation: Pergoli, Roberto; Kubicek, Karel; Hobor, Fruzsina; Pasulka, Josef; Stefl, Richard. "1H, 13C, and 15N chemical shift assignments for the RNA recognition motif of Nab3." Biomol. NMR Assignments 4, 119-121 (2010).
PubMed: 20309651

Assembly members:

Assembly members:
RNA_binding_polypeptide, polymer, 104 residues, Formula weight is not available

Natural source:

Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET22b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RNA_binding_polypeptide: MTEMHNIPPKSRLFIGNLPL KNVSKEDLFRIFSPYGHIMQ INIKNAFGFIQFDNPQSVRD AIECESQEMNFGKKLILEVS SSNARPQFDHGDHGTNLEHH HHHH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	201
15N chemical shifts	60
1H chemical shifts	376

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	subunit 1	1

Entities:

Entity 1, subunit 1 104 residues - Formula weight is not available

1

MET

THR

GLU

MET

HIS

ASN

ILE

PRO

LYS

2

SER

ARG

LEU

PHE

ILE

GLY

ASN

LEU

PRO

LEU

3

LYS

ASN

VAL

SER

LYS

GLU

ASP

LEU

PHE

ARG

4

ILE

PHE

SER

PRO

TYR

GLY

HIS

ILE

MET

GLN

5

ILE

ASN

ILE

LYS

ASN

ALA

PHE

GLY

PHE

ILE

6

GLN

PHE

ASP

ASN

PRO

GLN

SER

VAL

ARG

ASP

7

ALA

ILE

GLU

CYS

GLU

SER

GLN

GLU

MET

ASN

8

PHE

GLY

LYS

LEU

ILE

LEU

GLU

VAL

SER

9

SER

ASN

ALA

ARG

PRO

GLN

PHE

ASP

HIS

10

GLY

ASP

HIS

GLY

THR

ASN

LEU

GLU

HIS

11

HIS

Samples:

sample_1: RNA binding polypeptide, [U-99% 13C; U-99% 15N], 3 mM

sample_conditions_1: ionic strength: 0.3 M; pH: 8; pressure: 1 atm; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNHA	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN, Bruker Biospin, Goddard - chemical shift assignment, collection

NMR spectrometers:

Bruker DRX 600 MHz
Bruker Avance 900 MHz

PDB	2KVI 2L41 2XNQ 2XNR
DBJ	BAA07154 GAA26786
EMBL	CAA97903 CAY86769
GB	AAA81910 AHY77991 AJP41959 AJU23403 AJU24091
PRF	2201484A
REF	NP_015134
SP	P38996
TPG	DAA11244
AlphaFold	P38996