BMRB Entry 16549

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16549
MolProbity Validation Chart

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Title:
Solution NMR structure of the Mus81 N-terminal HhH. Northeast Structural Genomics Consortium target MmT1A
Deposition date:
2009-10-07
Original release date:
2009-11-09
Authors:
Laister, Rob; Wu, Bin; Lemak, Alexander; Arrowsmith, Cheryl
Citation:

Citation: Laister, Rob; Duan, Shili; Wu, Bin; Herzanych, Natalie; Arrowsmith, Cheryl. "Solution NMR structure and DNA binding mode of the Mus81 n-terminal helix-hairpin-helix domain."  .

Assembly members:

Assembly members:
Mus81_NTD, polymer, 80 residues, 9415.062 Da.

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGex-2Tk

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Mus81_NTD: RPLPVCPNPPFVRWLTEWRD EAASRGRHTRFVFQKALRSL QRYPLPLRSGKEAKILQHFG DRLCRMLDEKLKQHLASGGD

Data typeCount
13C chemical shifts258
15N chemical shifts73
1H chemical shifts524

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Mus81_NTD1

Entities:

Entity 1, Mus81_NTD 80 residues - 9415.062 Da.

1   ARGPROLEUPROVALCYSPROASNPROPRO
2   PHEVALARGTRPLEUTHRGLUTRPARGASP
3   GLUALAALASERARGGLYARGHISTHRARG
4   PHEVALPHEGLNLYSALALEUARGSERLEU
5   GLNARGTYRPROLEUPROLEUARGSERGLY
6   LYSGLUALALYSILELEUGLNHISPHEGLY
7   ASPARGLEUCYSARGMETLEUASPGLULYS
8   LEULYSGLNHISLEUALASERGLYGLYASP

Samples:

sample_1: Mus81 NTD, [U-100% 13C; U-100% 15N], 1.0 mM; sodium phosphate 25 mM; sodium chloride 500 mM; DTT 2 mM; benzamidine 2 mM; PMSF 0.5 mM; EDTA 0.5 mM; TCEP 0.5 mM

sample_2: Mus81 NTD, [U-100% 13C; U-100% 15N], 1 mM; sodium phosphate 25 mM; sodium chloride 500 mM; benzamidine 2 mM; DTT 2 mM; EDTA 0.5 mM; PMSF 0.5 mM; TCEP 0.5 mM

sample_conditions_1: ionic strength: 500 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D CCH-TOCSYsample_2isotropicsample_conditions_1
3D HCH-TOCSYsample_2isotropicsample_conditions_1
3D 1H-13C NOESYsample_2isotropicsample_conditions_1
3D 1H-13C NOESY arosample_2isotropicsample_conditions_1
CB-HDsample_2isotropicsample_conditions_1
CB-HEsample_2isotropicsample_conditions_1

Software:

NMRPipe v2.3, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

MDDGUI v1.0, Gutmanas and Arrowsmith - processing

XEASY, Bartels et al. - chemical shift assignment

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

PSVS, Bhattacharya and Montelione - NMR structure quality assessment

NMR spectrometers:

  • Varian INOVA 500 MHz
  • Bruker Avance 800 MHz
  • Varian INOVA 600 MHz

Related Database Links:

PDB
GB AAH26560 AAL28066 EDL33143
REF NP_082153 XP_006531901
SP Q91ZJ0
AlphaFold Q91ZJ0

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