BMRB Entry 16544

Title:
RECQL4 AMINO-TERMINAL DOMAIN
Deposition date:
2009-10-07
Original release date:
2012-08-03
Authors:
Ohlenschlager, Oliver; Gorlach, Matthias; Pospiech, Helmut
Citation:

Citation: Ohlenschlager, Oliver; Kuhnert, Anja; Schneider, Annerose; Haumann, Sebastian; Bellstedt, Peter; Keller, Heidi; Saluz, Hans-Peter; Hortschansky, Peter; Hanel, Frank; Grosse, Frank; Gorlach, Matthias; Pospiech, Helmut. "The N-terminus of the human RecQL4 helicase is a homeodomain-like DNA interaction motif."  Nucleic Acids Res. 40, 8309-8324 (2012).
PubMed: 22730300

Assembly members:

Assembly members:
RECQL4NT, polymer, 56 residues, 6850.708 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX1lambdaT

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts243
15N chemical shifts52
1H chemical shifts377

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RECQL4NT1

Entities:

Entity 1, RECQL4NT 56 residues - 6850.708 Da.

Residues 1-2 are non-native

1   GLYSERMETGLUARGLEUARGASPVALARG
2   GLUARGLEUGLNALATRPGLUARGALAPHE
3   ARGARGGLNARGGLYARGARGPROSERGLN
4   ASPASPVALGLUALAALAPROGLUGLUTHR
5   ARGALALEUTYRARGGLUTYRARGTHRLEU
6   LYSARGTHRTHRGLYGLN

Samples:

sample_1: RECQL4NT, [U-99% 15N], 1.2 mM; TRIS, [U-2H], 20 mM; sodium chloride 100 mM

sample_2: RECQL4NT, [U-99% 13C; U-99% 15N], 1.0 mM; TRIS, [U-2H], 20 mM; sodium chloride 100 mM

sample_conditions_1: ionic strength: 0.100 M; pH: 7.4; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D H(CCO)NHsample_2isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
3D HCCH-COSYsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_2isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

CARA, Keller and Wuthrich - chemical shift assignment

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 750 MHz

Related Database Links:

PDB
DBJ BAA74453 BAA86899
GB AAZ85145 EAW82071
REF NP_004251 XP_004047747 XP_011515682 XP_011515683 XP_011515684
SP O94761
AlphaFold O94761

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks