BMRB Entry 16539
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16539
MolProbity Validation Chart
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NMR-STAR v3 text file.
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Citation: Tian, Caihuan; Kouno, Takahide; Luo, Lan; Mizuguchi, Mineyuki; Zhu, Shunyi. "Not known" .
Assembly members:
nasonin-1, polymer, 33 residues, 3566.036 Da.
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
nasonin-1: ACNDRDCSLDCIMKGYNTGS
CVRGSCQCRRTSG
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 185 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | nasonin-1 | 1 |
Entities:
Entity 1, nasonin-1 33 residues - 3566.036 Da.
| 1 | ALA | CYS | ASN | ASP | ARG | ASP | CYS | SER | LEU | ASP | ||||
| 2 | CYS | ILE | MET | LYS | GLY | TYR | ASN | THR | GLY | SER | ||||
| 3 | CYS | VAL | ARG | GLY | SER | CYS | GLN | CYS | ARG | ARG | ||||
| 4 | THR | SER | GLY |
Samples:
sample_1: Nasonin-10.25 – 0.30 mM; acetic acid, [U-100% 2H], 50 mM; sodium chloride 20 mM; D2O, [U-100% 2H], 5%; H2O 95%
sample_2: Nasonin-10.25 – 0.30 mM; acetic acid, [U-100% 2H], 50 mM; sodium chloride 20 mM; D2O, [U-100% 2H], 100%
sample_conditions_1: ionic strength: 50 mM; pH: 3.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D DQF-COSY | sample_2 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_2 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_2 | isotropic | sample_conditions_1 |
Software:
xwinnmr, Bruker Biospin - collection
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
SPARKY, Goddard - peak picking
X-PLOR, Brunger - structure solution
NMR spectrometers:
- Bruker Avance 800 MHz