BMRB Entry 16537

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16537

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Title:
Backbone assignment of the 26 kDa neuron-specific ubiquitin carboxyl-terminal hydrolase L1 (UCH-L1)
Deposition date:
2009-10-04
Original release date:
2009-12-18
Authors:
Andersson, Fredrik; Jackson, Sophie; Hsu, Shang-Te Danny
Citation:

Citation: Andersson, Fredrik; Jackson, Sophie; Hsu, Shang-Te Danny. "Backbone assignments of the 26 kDa neuron-specific ubiquitin carboxyl-terminal hydrolase L1 (UCH-L1)."  Biomol. NMR Assignments 4, 41-43 (2010).
PubMed: 20012716

Assembly members:

Assembly members:
UCH-L1, polymer, 223 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX

Entity Sequences (FASTA):

Entity Sequences (FASTA):
UCH-L1: MQLKPMEINPEMLNKVLSRL GVAGQWRFVDVLGLEEESLG SVPAPACALLLLFPLTAQHE NFRKKQIEELKGQEVSPKVY FMKQTIGNSCGTIGLIHAVA NNQDKLGFEDGSVLKQFLSE TEKMSPEDRAKCFEKNEAIQ AAHDAVAQEGQCRVDDKVNF HFILFNNVDGHLYELDGRMP FPVNHGASSEDTLLKDAAKV CREFTEREQGEVRFSAVALC KAA

Data sets:
Data typeCount
13C chemical shifts597
15N chemical shifts203
1H chemical shifts295

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1UCH-L11

Entities:

Entity 1, UCH-L1 223 residues - Formula weight is not available

1   METGLNLEULYSPROMETGLUILEASNPRO
2   GLUMETLEUASNLYSVALLEUSERARGLEU
3   GLYVALALAGLYGLNTRPARGPHEVALASP
4   VALLEUGLYLEUGLUGLUGLUSERLEUGLY
5   SERVALPROALAPROALACYSALALEULEU
6   LEULEUPHEPROLEUTHRALAGLNHISGLU
7   ASNPHEARGLYSLYSGLNILEGLUGLULEU
8   LYSGLYGLNGLUVALSERPROLYSVALTYR
9   PHEMETLYSGLNTHRILEGLYASNSERCYS
10   GLYTHRILEGLYLEUILEHISALAVALALA
11   ASNASNGLNASPLYSLEUGLYPHEGLUASP
12   GLYSERVALLEULYSGLNPHELEUSERGLU
13   THRGLULYSMETSERPROGLUASPARGALA
14   LYSCYSPHEGLULYSASNGLUALAILEGLN
15   ALAALAHISASPALAVALALAGLNGLUGLY
16   GLNCYSARGVALASPASPLYSVALASNPHE
17   HISPHEILELEUPHEASNASNVALASPGLY
18   HISLEUTYRGLULEUASPGLYARGMETPRO
19   PHEPROVALASNHISGLYALASERSERGLU
20   ASPTHRLEULEULYSASPALAALALYSVAL
21   CYSARGGLUPHETHRGLUARGGLUGLNGLY
22   GLUVALARGPHESERALAVALALALEUCYS
23   LYSALAALA

Samples:

sample_1: UCH-L1, [U-98% 13C; U-98% 15N], 0.2 – 0.4 mM; TRIS 50 mM; EDTA 0.5 mM; DTT 5 mM

sample_conditions_1: ionic strength: . M; pH: 7.6; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 500 MHz
  • Bruker Avance 700 MHz
  • Varian INOVA 600 MHz
  • Varian INOVA 800 MHz

Related Database Links:

BMRB 17260
PDB
DBJ BAA28214 BAB33087 BAD51987 BAE87282 BAG37761
EMBL CAA28443
GB AAD09172 AAH00332 AAH05117 AAH06305 AAY40923
REF NP_004172 XP_001097839 XP_002745940 XP_002814743 XP_003258669
SP P09936 Q60HC8 Q9GM50
AlphaFold P09936 Q60HC8 Q9GM50

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks