BMRB Entry 16534

Name: DICER LIKE protein
Published: 2010-02-08

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PDB ID: 2kou
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16534
MolProbity Validation Chart

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

DICER LIKE protein

Deposition date:

2009-09-30

Original release date:

2010-02-08

Authors:

Qin, Haina; Song, Jianxing; Yuan, Adam, Yu-Ren

Citation:

Citation: Qin, Haina; Chen, Fading; Huan, Xuelu; Machida, Satoru; Song, Jianxing; Yuan, Y. Adam. "Structure of the Arabidopsis thaliana DCL4 DUF283 domain reveals a noncanonical double-stranded RNA-binding fold for protein-protein interaction." RNA 16, 474-481 (2010).
PubMed: 20106953

Assembly members:

Assembly members:
DCL4_DUF283, polymer, 102 residues, Formula weight is not available

Natural source:

Natural source: Common Name: thale cress Taxonomy ID: 3702 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Arabidopsis thaliana

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: PET32a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
DCL4_DUF283: ISGGSSISMMYKYCSRLPHD EFFQPKPEFQFKPVDEFGGT ICRITLPANAPISEIESSLL PSTEAAKKDACLKAVHELHN LGVLNDFLLPDSKDEIEDEL SD

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	246
15N chemical shifts	85
1H chemical shifts	514

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	DCL4_DUF283	1

Entities:

Entity 1, DCL4_DUF283 102 residues - Formula weight is not available

1

ILE

SER

GLY

SER

ILE

SER

MET

2

TYR

LYS

TYR

CYS

SER

ARG

LEU

PRO

HIS

ASP

3

GLU

PHE

GLN

PRO

LYS

PRO

GLU

PHE

GLN

4

PHE

LYS

PRO

VAL

ASP

GLU

PHE

GLY

THR

5

ILE

CYS

ARG

ILE

THR

LEU

PRO

ALA

ASN

ALA

6

PRO

ILE

SER

GLU

ILE

GLU

SER

LEU

7

PRO

SER

THR

GLU

ALA

LYS

ASP

ALA

8

CYS

LEU

LYS

ALA

VAL

HIS

GLU

LEU

HIS

ASN

9

LEU

GLY

VAL

LEU

ASN

ASP

PHE

LEU

PRO

10

ASP

SER

LYS

ASP

GLU

ILE

GLU

ASP

GLU

LEU

11

SER

ASP

Samples:

sample_1: protein, [U-100% 13C; U-100% 15N], 1 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.02 M; pH: 6.3; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - refinement

NMR spectrometers:

Bruker Avance 800 MHz

PDB	2KOU
GB	AAZ80387 AED92830 AED92831
REF	NP_001190348 NP_197532
SP	P84634
AlphaFold	P84634