BMRB Entry 16504

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR16504
MolProbity Validation Chart

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Title:
Human cannabinoid receptor 1 - helix 7/8 peptide
Deposition date:
2009-09-18
Original release date:
2009-11-20
Authors:
Deshmukh, Lalit; Vinogradova, Olga; Makriyannis, Alexandros; Tiburu, Elvis; Tyukhtenko, Sergiy; Janero, David
Citation:

Citation: Tyukhtenko, Sergiy; Tiburu, Elvis; Deshmukh, Lalit; Vinogradova, Olga; Janero, David; Makriyannis, Alexandros. "NMR solution structure of human cannabinoid receptor-1 helix 7/8 peptide: candidate electrostatic interactions and microdomain formation."  Biochem. Biophys. Res. Commun. 390, 441-446 (2009).
PubMed: 19766594

Assembly members:

Assembly members:
Human cannabinoid receptor 1, polymer, 40 residues, 4524.348 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Human cannabinoid receptor 1: TVFAFASMLCLLNSTVNPII YALRSKDLRHAFRSMFPSAE

Data sets:
Data typeCount
1H chemical shifts260

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Human cannabinoid receptor 11

Entities:

Entity 1, Human cannabinoid receptor 1 40 residues - 4524.348 Da.

1   THRVALPHEALAPHEALASERMETLEUCYS
2   LEULEUASNSERTHRVALASNPROILEILE
3   TYRALALEUARGSERLYSASPLEUARGHIS
4   ALAPHEARGSERMETPHEPROSERALAGLU

Samples:

sample_1: entity 1 mM; H2O 70%; TFE 30%

sample_conditions_1: pressure: 1 atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

AUREMOL, Bruker Biospin - data analysis

X-PLOR NIH v2.21, Schwieters, Kuszewski, Tjandra and Clore - structure solution

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

NMR spectrometers:

  • Bruker Avance 700 MHz

Related Database Links:

PDB
EMBL CDQ70034
GB AAY21179 EGV93809
REF XP_013999659