BMRB Entry 16492

Click here to enlarge.

PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16492
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
The Structure of Anti-TRAP
Deposition date:
2009-09-12
Original release date:
2010-10-18
Authors:
McElroy, Craig; Gollnick, Paul; Foster, Mark
Citation:

Citation: Sachleben, Joseph; McElroy, Craig; Gollnick, Paul; Foster, Mark. "Mechanism for pH-dependent gene regulation by amino-terminus-mediated homooligomerization of Bacillus subtilis anti-trp RNA-binding attenuation protein."  Proc. Natl. Acad. Sci. U.S.A. 107, 15385-15390 (2010).
PubMed: 20713740

Assembly members:

Assembly members:
Anti-TRAP, polymer, 53 residues, 5683.610 Da.
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Bacillus subtilis   Taxonomy ID: 1423   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bacillus subtilis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet24a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Anti-TRAP: XVIATDDLEVACPKCERAGE IEGTPCPACSGKGVILTAQG YTLLDFIQKHLNK

Data sets:
Data typeCount
13C chemical shifts223
15N chemical shifts53
1H chemical shifts352

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_1_11
2entity_1_21
3entity_1_31
4ZINC ION ON 3-FOLD CRYSTAL AXIS_12
5ZINC ION ON 3-FOLD CRYSTAL AXIS_22
6ZINC ION ON 3-FOLD CRYSTAL AXIS_32

Entities:

Entity 1, entity_1_1 53 residues - 5683.610 Da.

1   FMEVALILEALATHRASPASPLEUGLUVAL
2   ALACYSPROLYSCYSGLUARGALAGLYGLU
3   ILEGLUGLYTHRPROCYSPROALACYSSER
4   GLYLYSGLYVALILELEUTHRALAGLNGLY
5   TYRTHRLEULEUASPPHEILEGLNLYSHIS
6   LEUASNLYS

Entity 2, ZINC ION ON 3-FOLD CRYSTAL AXIS_1 - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: entity_1, [U-13C; U-15N], 5 – 10 mM; TRIS, [U-2H], 20 mM; beta-mercaptoethanol 20 mM; ZnCl2 1 mM; H2O 90%; D2O 10%

sample_2: entity_1, [U-13C; U-15N], 5 – 10 mM; TRIS, [U-2H], 20 mM; beta-mercaptoethanol 20 mM; ZnCl2 1 mM; D2O 100%

sample_conditions_1: ionic strength: 20 mM; pH: 7.5; pressure: 1 atm; temperature: 328 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D HCCH-COSYsample_2isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1
3D HNHBsample_1isotropicsample_conditions_1
3D HACAHB-COSYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_2isotropicsample_conditions_1
3D 1H-15N/13C TIME-SHARED NOESYsample_1isotropicsample_conditions_1
2D HCCH-COSY AROMATICsample_2isotropicsample_conditions_1
2D 1H-13C HSQC AROMATICsample_2isotropicsample_conditions_1
2D IPAPsample_1isotropicsample_conditions_1
2D 15N HETERONUCLEAR NOEsample_1isotropicsample_conditions_1
2D 15N HETERONUCLEAR T2sample_1isotropicsample_conditions_1
2D 15N HETERONUCLEAR T1sample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - data analysis

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution

ARIA, Linge, O'Donoghue and Nilges - refinement

NMR spectrometers:

  • Bruker DRX 800 MHz
  • Bruker DRX 600 MHz

Related Database Links:

PDB
DBJ BAI83705 BAM49183 BAM56453 GAK80258
EMBL CAB12047 CCU56704 CEI55371 CEJ75796 CJR84280
GB ADV95181 AEP89314 AFI26795 AFQ56187 AGA22564
REF NP_388135 WP_003234807 WP_014662639 WP_015382665 WP_015715179
SP O31466
AlphaFold O31466

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks