BMRB Entry 16484

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16484
MolProbity Validation Chart

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Title:
NMR structure of CIP75 UBA domain
Deposition date:
2009-09-08
Original release date:
2010-02-04
Authors:
Kieken, Fabien; Spagnol, Gaelle; Su, Vivian; Lau, Alan; Sorgen, Paul
Citation:

Citation: Kieken, Fabien; Spagnol, Gaelle; Su, Vivian; Lau, Alan; Sorgen, Paul. "NMR structure note: UBA domain of CIP75."  J. Biomol. NMR 46, 245-250 (2010).
PubMed: 20127391

Assembly members:

Assembly members:
CIP75_UBA, polymer, 53 residues, 5698.4 Da.

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-6P-2

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CIP75_UBA: GPLGSMPEVRFQQQLEQLNS MGFINREANLQALIATGGDI NAAIERLLGSQLS

Data sets:
Data typeCount
13C chemical shifts162
15N chemical shifts58
1H chemical shifts379

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1CIP75_UBA1

Entities:

Entity 1, CIP75_UBA 53 residues - 5698.4 Da.

Residues 1-5 represent a non-native cloning sequence.

1   GLYPROLEUGLYSERMETPROGLUVALARG
2   PHEGLNGLNGLNLEUGLUGLNLEUASNSER
3   METGLYPHEILEASNARGGLUALAASNLEU
4   GLNALALEUILEALATHRGLYGLYASPILE
5   ASNALAALAILEGLUARGLEULEUGLYSER
6   GLNLEUSER

Samples:

sample_1: CIP75 UBA, [U-13C; U-15N], 1.5-2.0 mM; D2O 7%; H2O 93%

sample_conditions_1: pH: 5.8; pressure: 1 atm; temperature controller setting: 280 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - chemical shift assignment, data analysis, peak picking

ARIA, Linge, O'Donoghue and Nilges - geometry optimization, refinement, structure solution

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
DBJ BAB40326 BAC36837 BAD92803 BAG37034 BAG64068
GB AAF19084 AAF80171 AAH06410 AAH17686 AAH18403
REF NP_001291271 NP_064516 NP_277068 XP_001117405 XP_001163250
SP Q99NB8 Q9NRR5
AlphaFold Q99NB8 Q9NRR5

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks