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BMRB Entry 16481 BMRB - Biological Magnetic Resonance Bank

BMRB Entry 16481

Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR16481

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Title:
1H, 13C and 15N resonance assignments of the hyperthermostable 264-residue endo-<beta>-1,3-glucanase LamA at 62 C
Deposition date:
2009-09-07
Original release date:
2009-11-20
Authors:
Ippel, Johannes; Koutsopoulos, Sotirios; Nabuurs, Sanne; van Berkel, Willem; van der Oost, John; van Mierlo, Carlo
Citation:

Citation: Ippel, Johannes; Koutsopoulos, Sotirios; Nabuurs, Sanne; van Berkel, Willem; van der Oost, John; van Mierlo, Carlo. "NMR characterization of a 264-residue hyperthermostable endo-beta-1,3-glucanase."  Biochem. Biophys. Res. Commun. 391, 370-375 (2010).
PubMed: 19913513

Assembly members:

Assembly members:
pfLamA, polymer, 264 residues, 30085 Da.
CA, non-polymer, 40.078 Da.

Natural source:

Natural source:   Common Name: Pyrococcus furiosus   Taxonomy ID: 2261   Superkingdom: Archaea   Kingdom: not available   Genus/species: Pyrococcus furiosus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: DE3

Entity Sequences (FASTA):

Entity Sequences (FASTA):
pfLamA: MVPEVIEIDGKQWRLIWHDE FEGSEVNKEYWTFEKGNGIA YGIPGWGNGELEYYTENNTY IVNGTLVIEARKEIITDPNE GTFLYTSSRLKTEGKVEFSP PVVVEARIKLPKGKGLWPAF WMLGSNIREVGWPNCGEIDI MEFLGHEPRTIHGTVHGPGY SGSKGITRAYTLPEGVPDFT EDFHVFGIVWYPDKIKWYVD GTFYHEVTKEQVEAMGYEWV FDKPFYIILNLAVGGYWPGN PDATTPFPAKMVVDYVRVYS FVSG

Data typeCount
13C chemical shifts1799
15N chemical shifts526
1H chemical shifts1961

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