BMRB Entry 16479

Name: 1H and 13C resonance assignments of a guanine sensing riboswitch&#039;s terminator hairpin
Published: 2010-03-15

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16479

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H and 13C resonance assignments of a guanine sensing riboswitch's terminator hairpin

Deposition date:

2009-09-04

Original release date:

2010-03-15

Authors:

Ottink, Otmar; Westerweele, Ivo; Nelissen, Frank; Tessari, Marco; Heus, Hans; Wijmenga, Sybren

Citation:

Citation: Ottink, Otmar; Westerweele, Ivo; Tesssari, Marco; Nelissen, Frank; Heus, Hans; Wijmenga, Sybren. "(1)H and (13)C resonance assignments of a guanine sensing riboswitch's terminator hairpin." Biomol. NMR Assignments 4, 89-91 (2010).
PubMed: 20204714

Assembly members:

Assembly members:
G-riboswitch_terminator_hairpin, polymer, 35 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Bacillus subtilis Taxonomy ID: 1423 Superkingdom: Bacteria Kingdom: not available Genus/species: Bacillus subtilis

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pUC18

Entity Sequences (FASTA):

Entity Sequences (FASTA):
G-riboswitch_terminator_hairpin: GGCAUUGCUUGCUCUUUAUU UGAGCGGGCAAUGCC

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	90
1H chemical shifts	119

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	subunit 1	1

Entities:

Entity 1, subunit 1 35 residues - Formula weight is not available

1

G

C

A

U

G

C

U

2

G

C

U

C

U

A

U

3

U

G

A

G

C

G

C

A

4

A

U

G

C

Samples:

sample_1: G-riboswitch terminator hairpin 0.44 mM; D2O 7%; H2O 93%; NaPhosphate buffer (ph 6.7) 10 mM

sample_2: G-riboswitch terminator hairpin 0.44 mM; D2O 100%; NaPhosphate buffer (ph 6.7) 10 mM

sample_conditions_1: ionic strength: 10 mM; pH: 6.7; pressure: 1 atm; temperature: 288 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_2	isotropic	sample_conditions_1
2D DQF-COSY	sample_2	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_2	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment, peak picking

NMR spectrometers:

Varian INOVA 800 MHz