BMRB Entry 16460

Name: The B12-Binding Subunit of Glutamate Mutase from Clostridium tetanomorphum Traps the Nucleotide Moiety of Coenzyme B12
Published: 2010-03-08

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16460

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

The B12-Binding Subunit of Glutamate Mutase from Clostridium tetanomorphum Traps the Nucleotide Moiety of Coenzyme B12

Deposition date:

2009-08-19

Original release date:

2010-03-08

Authors:

Tollinger, Martin; Eichmuller, Chritian; Konrat, Robert; Huhta, Maria; Marsh, E.; Krautler, Bernhard

Citation:

Citation: Tollinger, Martin; Eichmuller, Chritian; Konrat, Robert; Huhta, Maria; Marsh, E.; Krautler, Bernhard. "The B(12)-binding subunit of glutamate mutase from Clostridium tetanomorphum traps the nucleotide moiety of coenzyme B(12).Moiety of Coenzyme B12" J. Mol. Biol. 309, 777-791 (2001).
PubMed: 11397096

Assembly members:

Assembly members:
MutS, polymer, 136 residues, Formula weight is not available
B12-nucleotide, non-polymer, Formula weight is not available

Natural source:

Natural source: Common Name: Clostridium tetanomorphum Taxonomy ID: 1553 Superkingdom: Bacteria Kingdom: not available Genus/species: Clostridium tetanomorphum

Experimental source:

Experimental source: Production method: purified from the natural source

Entity Sequences (FASTA):

Entity Sequences (FASTA):
MutS: MEKKTIVLGVIGSDCHAVGN KILDHSFTNAGFNVVNIGVL SSQEDFINAAIETKADLICV SSLYGQGEIDCKGLREKCDE AGLKGIKLFVGGNIVVGKQN WPDVEQRFKAMGFDRVYPPG TSPETTIADMKEVLGV

Data sets:

binding_constants

Data type	Count
binding constants	1

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	MutS	1
2	B12-nucleotide	2

Entities:

Entity 1, MutS 136 residues - Formula weight is not available

1

MET

GLU

LYS

THR

ILE

VAL

LEU

GLY

VAL

2

ILE

GLY

SER

ASP

CYS

HIS

ALA

VAL

GLY

ASN

3

LYS

ILE

LEU

ASP

HIS

SER

PHE

THR

ASN

ALA

4

GLY

PHE

ASN

VAL

ASN

ILE

GLY

VAL

LEU

5

SER

GLN

GLU

ASP

PHE

ILE

ASN

ALA

6

ILE

GLU

THR

LYS

ALA

ASP

LEU

ILE

CYS

VAL

7

SER

LEU

TYR

GLY

GLN

GLY

GLU

ILE

ASP

8

CYS

LYS

GLY

LEU

ARG

GLU

LYS

CYS

ASP

GLU

9

ALA

GLY

LEU

LYS

GLY

ILE

LYS

LEU

PHE

VAL

10

GLY

ASN

ILE

VAL

GLY

LYS

GLN

ASN

11

TRP

PRO

ASP

VAL

GLU

GLN

ARG

PHE

LYS

ALA

12

MET

GLY

PHE

ASP

ARG

VAL

TYR

PRO

GLY

13

THR

SER

PRO

GLU

THR

ILE

ALA

ASP

MET

14

LYS

GLU

VAL

LEU

GLY

VAL

Entity 2, B12-nucleotide - C16 H26 N3 O7 P - Formula weight is not available

Samples:

sample_1: phosphate buffer 11 mM; DTT 5 mM; MutS, [U-15N], 0.8 mM; B12-nucleotide0.4 – 25.6 mM; D2O 10%; H2O 90%

sample_conditions_1: pH: 6.0; pressure: 1 atm; temperature: 299 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1

Software:

Molmol, Koradi, Billeter and Wuthrich - visualization

X-PLOR v3.1, Brunger - structure solution

NMR spectrometers:

Varian UnityPlus 500 MHz

PDB	1BE1 1FMF 1ID8
EMBL	CAA48567 CAA49908
GB	KAJ52263
PRF	1907157B
REF	WP_027625115 WP_035147515
SP	Q05488
AlphaFold	Q05488