BMRB Entry 16460

Title:
The B12-Binding Subunit of Glutamate Mutase from Clostridium tetanomorphum Traps the Nucleotide Moiety of Coenzyme B12
Deposition date:
2009-08-19
Original release date:
2010-03-08
Authors:
Tollinger, Martin; Eichmuller, Chritian; Konrat, Robert; Huhta, Maria; Marsh, E.; Krautler, Bernhard
Citation:

Citation: Tollinger, Martin; Eichmuller, Chritian; Konrat, Robert; Huhta, Maria; Marsh, E.; Krautler, Bernhard. "The B(12)-binding subunit of glutamate mutase from Clostridium tetanomorphum traps the nucleotide moiety of coenzyme B(12).Moiety of Coenzyme B12"  J. Mol. Biol. 309, 777-791 (2001).
PubMed: 11397096

Assembly members:

Assembly members:
MutS, polymer, 136 residues, Formula weight is not available
B12-nucleotide, non-polymer, Formula weight is not available

Natural source:

Natural source:   Common Name: Clostridium tetanomorphum   Taxonomy ID: 1553   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Clostridium tetanomorphum

Experimental source:

Experimental source:   Production method: purified from the natural source

Data sets:
  • binding_constants
Data typeCount
binding constants1

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MutS1
2B12-nucleotide2

Entities:

Entity 1, MutS 136 residues - Formula weight is not available

1   METGLULYSLYSTHRILEVALLEUGLYVAL
2   ILEGLYSERASPCYSHISALAVALGLYASN
3   LYSILELEUASPHISSERPHETHRASNALA
4   GLYPHEASNVALVALASNILEGLYVALLEU
5   SERSERGLNGLUASPPHEILEASNALAALA
6   ILEGLUTHRLYSALAASPLEUILECYSVAL
7   SERSERLEUTYRGLYGLNGLYGLUILEASP
8   CYSLYSGLYLEUARGGLULYSCYSASPGLU
9   ALAGLYLEULYSGLYILELYSLEUPHEVAL
10   GLYGLYASNILEVALVALGLYLYSGLNASN
11   TRPPROASPVALGLUGLNARGPHELYSALA
12   METGLYPHEASPARGVALTYRPROPROGLY
13   THRSERPROGLUTHRTHRILEALAASPMET
14   LYSGLUVALLEUGLYVAL

Entity 2, B12-nucleotide - C16 H26 N3 O7 P - Formula weight is not available

Samples:

sample_1: phosphate buffer 11 mM; DTT 5 mM; MutS, [U-15N], 0.8 mM; B12-nucleotide0.4 – 25.6 mM; D2O 10%; H2O 90%

sample_conditions_1: pH: 6.0; pressure: 1 atm; temperature: 299 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

Molmol, Koradi, Billeter and Wuthrich - visualization

X-PLOR v3.1, Brunger - structure solution

NMR spectrometers:

  • Varian UnityPlus 500 MHz

Related Database Links:

PDB
EMBL CAA48567 CAA49908
GB KAJ52263
PRF 1907157B
REF WP_027625115 WP_035147515
SP Q05488
AlphaFold Q05488