BMRB Entry 16455

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16455

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Title:
Study of the interaction between salivary proline-rich proteins and a polyphenol by 1H-NMR spectroscopy
Deposition date:
2009-08-19
Original release date:
2010-03-08
Authors:
Murray, Nicola; Williamson, Michael; Lilley, Terence; Haslam, Edwin
Citation:

Citation: Murray, Nicola; Williamson, Michael; Lilley, Terence; Haslam, Edwin. "Study of the interaction between salivary proline-rich proteins and a polyphenol by 1H-NMR spectroscopy"  Eur. J. Biochem. 219, 923-935 (1994).
PubMed: 8112344

Assembly members:

Assembly members:
Peptide_2, polymer, 22 residues, Formula weight is not available
BETA-1,2,3,4,6-PENTA-O-GALLOYL-D-GLUCOPYRANOSE, non-polymer, 940.677 Da.

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: purified from the natural source

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Peptide_2: GPQQRPPQPGNQQGPPPQGG PQ

Data sets:
  • binding_constants
Data typeCount
binding constants19

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Peptide_21
2Gall5Glc2

Entities:

Entity 1, Peptide_2 22 residues - Formula weight is not available

1   GLYPROGLNGLNARGPROPROGLNPROGLY
2   ASNGLNGLNGLYPROPROPROGLNGLYGLY
3   PROGLN

Entity 2, Gall5Glc - C41 H32 O26 - 940.677 Da.

1   GGP

Samples:

sample_2: Peptide 2 2 mM; H2O 90%; DMSO 10%

sample_3: Gall5Glc .4 mM; Peptide 2 2 mM; H2O 90%; DMSO 10%

sample_4: Gall5Glc .78 mM; Peptide 2 1.95 mM; H2O 90%; DMSO 10%

sample_5: Gall5Glc 1.17 mM; Peptide 2 1.95 mM; H2O 90%; DMSO 10%

sample_6: Gall5Glc 1.54 mM; Peptide 2 1.92 mM; H2O 90%; DMSO 10%

sample_7: Gall5Glc 1.91 mM; Peptide 2 1.91 mM; H2O 90%; DMSO 10%

sample_8: Gall5Glc 2.26 mM; Peptide 2 1.88 mM; H2O 90%; DMSO 10%

sample_9: Gall5Glc 2.62 mM; Peptide 2 1.87 mM; H2O 90%; DMSO 10%

sample_10: Gall5Glc 2.96 mM; Peptide 2 1.85 mM; H2O 90%; DMSO 10%

sample_11: Gall5Glc 3.64 mM; Peptide 2 1.82 mM; H2O 90%; DMSO 10%

sample_12: Gall5Glc 4.29 mM; Peptide 2 1.79 mM; H2O 90%; DMSO 10%

sample_13: Gall5Glc 4.91 mM; Peptide 2 1.75 mM; H2O 90%; DMSO 10%

sample_14: Gall5Glc 5.52 mM; Peptide 2 1.72 mM; H2O 90%; DMSO 10%

sample_15: Gall5Glc 6.10 mM; Peptide 2 1.69 mM; H2O 90%; DMSO 10%

sample_16: Gall5Glc 6.67 mM; Peptide 2 1.67 mM; H2O 90%; DMSO 10%

sample_17: Gall5Glc 7.21 mM; Peptide 2 1.64 mM; H2O 90%; DMSO 10%

sample_18: Gall5Glc 7.74 mM; Peptide 2 1.61 mM; H2O 90%; DMSO 10%

sample_19: Gall5Glc 8.25 mM; Peptide 2 1.59 mM; H2O 90%; DMSO 10%

sample_20: Gall5Glc 8.75 mM; Peptide 2 1.56 mM; H2O 90%; DMSO 10%

sample_conditions_1: pH: 3.3; pressure: 1 atm; temperature: 276 K

Experiments:

NameSampleSample stateSample conditions
ROESYsample_7isotropicsample_conditions_1
2D 1H-1H TOCSYsample_2isotropicsample_conditions_1
2D 1H-1H TOCSYsample_3isotropicsample_conditions_1
2D 1H-1H TOCSYsample_4isotropicsample_conditions_1
2D 1H-1H TOCSYsample_5isotropicsample_conditions_1
2D 1H-1H TOCSYsample_6isotropicsample_conditions_1
2D 1H-1H TOCSYsample_7isotropicsample_conditions_1
2D 1H-1H TOCSYsample_8isotropicsample_conditions_1
2D 1H-1H TOCSYsample_9isotropicsample_conditions_1
2D 1H-1H TOCSYsample_10isotropicsample_conditions_1
2D 1H-1H TOCSYsample_11isotropicsample_conditions_1
2D 1H-1H TOCSYsample_12isotropicsample_conditions_1
2D 1H-1H TOCSYsample_13isotropicsample_conditions_1
2D 1H-1H TOCSYsample_14isotropicsample_conditions_1
2D 1H-1H TOCSYsample_15isotropicsample_conditions_1
2D 1H-1H TOCSYsample_16isotropicsample_conditions_1
2D 1H-1H TOCSYsample_17isotropicsample_conditions_1
2D 1H-1H TOCSYsample_18isotropicsample_conditions_1
2D 1H-1H TOCSYsample_19isotropicsample_conditions_1
2D 1H-1H TOCSYsample_20isotropicsample_conditions_1
2D 1H-1H NOESYsample_17isotropicsample_conditions_1
2D 1H-1H NOESYsample_20isotropicsample_conditions_1

Software:

FELIX, Accelrys Software Inc. - processing

NMR spectrometers:

  • Bruker AMX 500 MHz
  • Bruker AMX 600 MHz

Related Database Links:

EMBL CAA44733 CAA44734
GB AAI71935 AAI71936 ABR45898 EDL29792 EDL29793
REF NP_001171059 XP_006506909 XP_011236341