BMRB Entry 16453

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16453

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Title:
Intramolecular Dynamics of Low Molecular Weight Protein Tyrosine Phosphatase in Monomer-Dimer Equilibrium Studied by NMR: A Model for Changes in Dynamics upon Target Binding
Deposition date:
2009-08-19
Original release date:
2010-03-08
Authors:
Akerud, Tomas; Thulin, Eva; Van Etten, Robert; Akke, Mikael
Citation:

Citation: Akerud, Tomas; Thulin, Eva; Van Etten, Robert; Akke, Mikael. "Intramolecular Dynamics of Low Molecular Weight Protein Tyrosine Phosphatase in Monomer-Dimer Equilibrium Studied by NMR: A Model for Changes in Dynamics upon Target Binding"  J. Mol. Biol. 322, 137-152 (2002).
PubMed: 12215420

Assembly members:

Assembly members:
LMW-PTP, polymer, 157 residues, Formula weight is not available
PO4, non-polymer, 94.971 Da.

Natural source:

Natural source:   Common Name: cow   Taxonomy ID: 9913   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Bos taurus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-11d

Entity Sequences (FASTA):

Entity Sequences (FASTA):
LMW-PTP: AEQVTKSVLFVCLGNICRSP IAEAVFRKLVTDQNISDNWV IDSGAVSDWNVGRSPDPRAV SCLRNHGINTAHKARQVTKE DFVTFDYILCMDESNLRDLN RKSNQVKNCRAKIELLGSYD PQKQLIIEDPYYGNDADFET VYQQCVRCCRAFLEKVR

Data sets:
  • binding_constants
Data typeCount
binding constants6

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1LMW-PTP11
2LMW-PTP21
3Phosphate12
4Phosphate22

Entities:

Entity 1, LMW-PTP1 157 residues - Formula weight is not available

1   ALAGLUGLNVALTHRLYSSERVALLEUPHE
2   VALCYSLEUGLYASNILECYSARGSERPRO
3   ILEALAGLUALAVALPHEARGLYSLEUVAL
4   THRASPGLNASNILESERASPASNTRPVAL
5   ILEASPSERGLYALAVALSERASPTRPASN
6   VALGLYARGSERPROASPPROARGALAVAL
7   SERCYSLEUARGASNHISGLYILEASNTHR
8   ALAHISLYSALAARGGLNVALTHRLYSGLU
9   ASPPHEVALTHRPHEASPTYRILELEUCYS
10   METASPGLUSERASNLEUARGASPLEUASN
11   ARGLYSSERASNGLNVALLYSASNCYSARG
12   ALALYSILEGLULEULEUGLYSERTYRASP
13   PROGLNLYSGLNLEUILEILEGLUASPPRO
14   TYRTYRGLYASNASPALAASPPHEGLUTHR
15   VALTYRGLNGLNCYSVALARGCYSCYSARG
16   ALAPHELEUGLULYSVALARG

Entity 2, Phosphate1 - O4 P - 94.971 Da.

1   PO4

Samples:

sample_1: LMW-PTP, [U-100% 15N], 0.026 ± 0.002 mM; Phosphate 200 mM; DTT 10 mM; sodium azide 3 mM; D2O 10%; H2O 90%

sample_2: LMW-PTP, [U-100% 15N], 0.079 ± 0.005 mM; Phosphate 200 mM; DTT 10 mM; sodium azide 3 mM; D2O 10%; H2O 90%

sample_3: LMW-PTP, [U-100% 15N], 0.17 ± 0.02 mM; Phosphate 200 mM; DTT 10 mM; sodium azide 3 mM; D2O 10%; H2O 90%

sample_4: LMW-PTP, [U-100% 15N], 0.34 ± 0.03 mM; Phosphate 200 mM; DTT 10 mM; sodium azide 3 mM; D2O 10%; H2O 90%

sample_5: LMW-PTP, [U-100% 15N], 0.66 ± 0.04 mM; Phosphate 200 mM; DTT 10 mM; sodium azide 3 mM; D2O 10%; H2O 90%

sample_6: LMW-PTP, [U-100% 15N], 1.24 ± 0.07 mM; Phosphate 200 mM; DTT 10 mM; sodium azide 3 mM; D2O 10%; H2O 90%

sample_conditions_1: pH: 5.0; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
2D 1H-15N HSQCsample_3isotropicsample_conditions_1
2D 1H-15N HSQCsample_4isotropicsample_conditions_1
2D 1H-15N HSQCsample_5isotropicsample_conditions_1
2D 1H-15N HSQCsample_6isotropicsample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - data analysis

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

Molmol, Koradi, Billeter and Wuthrich - geometry optimization

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
GB AAC37328
REF NP_776403 XP_004004007 XP_006062677 XP_010818256 XP_010824218
SP P11064
TPG DAA33805
AlphaFold P11064