BMRB Entry 16439

Name: Combined high- and low-resolution techniques reveal compact structure in central portion of factor H despite long inter-modular linkers
Published: 2009-11-19

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PDB ID: 2kms
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16439
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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Combined high- and low-resolution techniques reveal compact structure in central portion of factor H despite long inter-modular linkers

Deposition date:

2009-08-04

Original release date:

2009-11-19

Authors:

Schmidt, Christoph; Herbert, Andrew; Guariento, Mara; Mertens, Haydyn; Soares, Dinesh; Uhrin, Dusan; Rowe, Arthur; Svergun, Dmitri; Barlow, Paul

Citation:

Citation: Schmidt, Christoph; Herbert, Andrew; Mertens, Haydyn; Guariento, Mara; Soares, Dinesh; Uhrin, Dusan; Rowe, Arthur; Svergun, Dmitri; Barlow, Paul. "The central portion of factor H (modules 10-15) is compact and contains a structurally deviant CCP module." J. Mol. Biol. 395, 105-122 (2010).
PubMed: 19835885

Assembly members:

Assembly members:
factor H (modules 10-15), polymer, 121 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Pichia pastoris Vector: pPICZalphaB

Entity Sequences (FASTA):

Entity Sequences (FASTA):
factor H (modules 10-15): EAEAAGTCGDIPELEHGWAQ LSSPPYYYGDSVEFNCSESF TMIGHRSITCIHGVWTQLPQ CVAIDKLKKCKSSNLIILEE HLKNKKEFDHNSNIRYRCRG KEGWIHTVCINGRWDPEVNC S

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	508
15N chemical shifts	127
1H chemical shifts	746

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	factor H (modules 10-15)	1

Entities:

Entity 1, factor H (modules 10-15) 121 residues - Formula weight is not available

1

GLU

ALA

GLU

ALA

GLY

THR

CYS

GLY

ASP

2

ILE

PRO

GLU

LEU

GLU

HIS

GLY

TRP

ALA

GLN

3

LEU

SER

PRO

TYR

GLY

ASP

4

SER

VAL

GLU

PHE

ASN

CYS

SER

GLU

SER

PHE

5

THR

MET

ILE

GLY

HIS

ARG

SER

ILE

THR

CYS

6

ILE

HIS

GLY

VAL

TRP

THR

GLN

LEU

PRO

GLN

7

CYS

VAL

ALA

ILE

ASP

LYS

LEU

LYS

CYS

8

LYS

SER

ASN

LEU

ILE

LEU

GLU

9

HIS

LEU

LYS

ASN

LYS

GLU

PHE

ASP

HIS

10

ASN

SER

ASN

ILE

ARG

TYR

ARG

CYS

ARG

GLY

11

LYS

GLU

GLY

TRP

ILE

HIS

THR

VAL

CYS

ILE

12

ASN

GLY

ARG

TRP

ASP

PRO

GLU

VAL

ASN

CYS

13

SER

Samples:

sample_1: potassium phosphate 20 mM; entity, [U-100% 13C; U-100% 15N], 0.62 mM; D2O 7%; H2O 93%

sample_conditions_1: ionic strength: 20 mM; pH: 6.6; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D CBCANH	sample_1	isotropic	sample_conditions_1

Software:

CNS v1.2, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

Molmol, Koradi, Billeter and Wuthrich - data analysis

AZARA, Boucher - processing

ProcheckNMR, Laskowski and MacArthur - data analysis

TOPSPIN, Bruker Biospin - collection

WhatIF, Vriend - data analysis

NMR spectrometers:

Bruker Avance 800 MHz
Bruker Avance 600 MHz