BMRB Entry 16420

Name: Protein stablilization by specific binding of guanidinium to a functional arginine-binding surface on an SH3 domain
Published: 2015-09-18

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16420

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Protein stablilization by specific binding of guanidinium to a functional arginine-binding surface on an SH3 domain

Deposition date:

2009-07-23

Original release date:

2015-09-18

Authors:

Zarrine-Afsar, Arash; Mittermaier, Anthony; Kay, Lewis; Davidson, Alan

Citation:

Citation: Zarrine-Afsar, Arash; Mittermaier, Anthony; Kay, Lewis; Davidson, Alan. "Protein stabilization by specific binding of guanidinium to a functional arginine-binding surface on an SH3 domain" Protein Sci. 1, 162-170 (2006).
PubMed: 16373478

Assembly members:

Assembly members:
Fyn_SH3_domain, polymer, 59 residues, 6733.33 Da.
GuHCl, non-polymer, 59.070 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: n/a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Fyn_SH3_domain: VTLFVALYDYEARTEDDLSF HKGEKFQILNSSEGDWWEAR SLTTGETGYIPSNYVAPVD

Data sets:

Data type

Count

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Fyn SH3 domain	1
2	Guanidium chloride	2

Entities:

Entity 1, Fyn SH3 domain 59 residues - 6733.33 Da.

1

VAL

THR

LEU

PHE

VAL

ALA

LEU

TYR

ASP

TYR

2

GLU

ALA

ARG

THR

GLU

ASP

LEU

SER

PHE

3

HIS

LYS

GLY

GLU

LYS

PHE

GLN

ILE

LEU

ASN

4

SER

GLU

GLY

ASP

TRP

GLU

ALA

ARG

5

SER

LEU

THR

GLY

GLU

THR

GLY

TYR

ILE

6

PRO

SER

ASN

TYR

VAL

ALA

PRO

VAL

ASP

Entity 2, Guanidium chloride - . - 59.070 Da.

1

.

Samples:

sample_1: Fyn SH3 domain, [U-100% 15N], 0.25 mM; sodium phosphate 50 mM; NaCl 100 mM; EDTA 0.2 mM; DSS 4 mM; GuHCl0 – 0.85 M

sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - peak picking

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - peak picking

Molmol, Koradi, Billeter and Wuthrich - processing

NMR spectrometers:

Varian INOVA 500 MHz

PDB	1shf 1A0N 1AVZ 1AZG 1EFN 1FYN 1G83 1M27 1NYF 1NYG 1SHF 1ZBJ 3H0F 3H0H 3H0I 3UA6 3UA7 3UF4 4D8D 4EIK
DBJ	BAE33766 BAE42585 BAG70107 BAG70240 BAI46902
EMBL	CAA37025 CAC44028
GB	AAA36615 AAA37644 AAA82942 AAB09568 AAB33113
REF	NP_001071440 NP_001073675 NP_001116364 NP_001116365 NP_002028
SP	A0JNB0 A1Y2K1 P06241 P39688 Q05876
TPG	DAA26259
AlphaFold	P06241 A0JNB0 A1Y2K1 P39688 Q05876