BMRB Entry 16419

Name: Local Perturbations by Ligand Binding of Hydrogen Deuterium Exchange Kinetics in a Four-helix Bundle Protein, Acyl Coenzyme A Binding Protein (ACBP)
Published: 2012-06-06

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16419

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Local Perturbations by Ligand Binding of Hydrogen Deuterium Exchange Kinetics in a Four-helix Bundle Protein, Acyl Coenzyme A Binding Protein (ACBP)

Deposition date:

2009-07-23

Original release date:

2012-06-06

Authors:

Kragelund, Birthe; Knudsen, Jens; Poulsen, Flemming

Citation:

Citation: Kragelund, B.; Knudsen, J.; Poulsen, F.. "Local perturbations by ligand binding of hydrogen deuterium exchange kinetics in a four-helix bundle protein, acyl coenzyme A binding protein (ACBP)." J. Mol. Biol. 250, 695-706 (1995).
PubMed: 7623386

Assembly members:

Assembly members:
ACBP, polymer, 86 residues, 10043.79 Da.
TH3, non-polymer, 1003.927 Da.

Natural source:

Natural source: Common Name: cow Taxonomy ID: 9913 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Bos taurus

Experimental source:

Experimental source: Production method: purified from the natural source

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ACBP: SQAEFDKAAEEVKHLKTKPA DEEMLFIYSHYKQATVGDIN TERPGMLDFKGKAKWDAWNE LKGTSKEDAMKAYIDKVEEL KKKYGI

Data sets:

chemical_rates
- Chemical_rate_list

Data type	Count
chemical rates	56

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	ACBP	1
2	palmitoyl-CoA	2

Entities:

Entity 1, ACBP 86 residues - 10043.79 Da.

1

SER

GLN

ALA

GLU

PHE

ASP

LYS

ALA

GLU

2

GLU

VAL

LYS

HIS

LEU

LYS

THR

LYS

PRO

ALA

3

ASP

GLU

MET

LEU

PHE

ILE

TYR

SER

HIS

4

TYR

LYS

GLN

ALA

THR

VAL

GLY

ASP

ILE

ASN

5

THR

GLU

ARG

PRO

GLY

MET

LEU

ASP

PHE

LYS

6

GLY

LYS

ALA

LYS

TRP

ASP

ALA

TRP

ASN

GLU

7

LEU

LYS

GLY

THR

SER

LYS

GLU

ASP

ALA

MET

8

LYS

ALA

TYR

ILE

ASP

LYS

VAL

GLU

LEU

9

LYS

TYR

GLY

ILE

Entity 2, palmitoyl-CoA - C37 H64 N7 O17 P3 S - 1003.927 Da.

1

TH3

Samples:

sample_1: ACBP 2 mM; potassium phosphate 0.04 mM; palmitoyl-CoA 2 mM; D2O 100%

sample_conditions_1: pH: 6.65; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1

Software:

PRONTO, Kjur, Andersen and Poulsen - data analysis, processing

MNMR - processing

NMR spectrometers:

Bruker AMX 600 MHz

BMRB	2049 2050
PDB	1ACA 1HB6 1HB8 1NTI 1NVL 2ABD
EMBL	CAA44618
GB	AAA30495 AAI14182 AIM41257 ELR45212
REF	NP_001106792 XP_004004799 XP_005910864 XP_006040065 XP_006057227
SP	P07107
TPG	DAA32604
AlphaFold	P07107