BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 16405

Title: Solution Structure of the Tandem UIM Domain of Ataxin-3 Complexed with Ubiquitin   PubMed: 20949063

Deposition date: 2009-07-12 Original release date: 2012-08-03

Authors: Zhou, Chenjie; Song, Aixin; Lin, Donghai; Hu, Hongyu

Citation: Song, Ai-Xin; Zhou, Chen-Jie; Peng, Yu; Gao, Xue-Chao; Zhou, Zi-Ren; Fu, Qing-Shan; Hong, Jing; Lin, Dong-Hai; Hu, Hong-Yu. "Structural Transformation of the Tandem Ubiquitin-Interacting Motifs in Ataxin-3 and Their Cooperative Interactions with Ubiquitin Chains"  PLoS ONE 5, e13202-e13202 (2010).

Assembly members:
Ataxin-3/Ubiquitin complex, polymer, 46 residues, 5268.689 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology' 'Escherichia coli

Entity Sequences (FASTA):
Ataxin-3/Ubiquitin complex: GSLDEDEEDLQRALALSRQE IDMEDEEADLRRAIQLSMQG SSRNLE

Data sets:
Data typeCount
13C chemical shifts133
15N chemical shifts41
1H chemical shifts241

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Ataxin-3/Ubiquitin complex1

Entities:

Entity 1, Ataxin-3/Ubiquitin complex 46 residues - 5268.689 Da.

1   GLYSERLEUASPGLUASPGLUGLUASPLEU
2   GLNARGALALEUALALEUSERARGGLNGLU
3   ILEASPMETGLUASPGLUGLUALAASPLEU
4   ARGARGALAILEGLNLEUSERMETGLNGLY
5   SERSERARGASNLEUGLU

Samples:

sample_1: entity, [U-100% 15N], 1 mM; sodium phosphate' 'natural abundance; .; . %; . mM

sample_2: entity, [U-100% 13C; U-100% 15N], 1 mM; sodium phosphate' 'natural abundance; .; . %; . mM

sample_conditions_1: ionic strength: 120 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_2isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D H(CCO)NHsample_2isotropicsample_conditions_1
3D C(CO)NHsample_2isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_2isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment, data analysis

ARIA, Linge, O'Donoghue and Nilges - refinement, structure solution

ProcheckNMR, Laskowski and MacArthur - data analysis

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
GB ADD00635 EHB02478 ELW55804
REF XP_004584394 XP_004681665 XP_004681676 XP_004837073 XP_009004709

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts