BMRB Entry 16399

Title:
Role of Trp-Trp interactions in Trpzip beta-hairpin formation, structure and stability
Deposition date:
2009-07-09
Original release date:
2009-08-12
Authors:
Wu, Ling; McElheny, Dan; Huang, Rong; Keiderling, Timothy
Citation:

Citation: Wu, Ling; McElheny, Dan; Huang, Rong; Keiderling, Timothy. "Role of tryptophan-tryptophan interactions in Trpzip beta-hairpin formation, structure, and stability."  Biochemistry 48, 10362-10371 (2009).
PubMed: 19788311

Assembly members:

Assembly members:
W4W9, polymer, 12 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
W4W9: SVTWENGKWTVK

Data sets:
Data typeCount
1H chemical shifts82

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1monomer1

Entities:

Entity 1, monomer 12 residues - Formula weight is not available

1   SERVALTHRTRPGLUASNGLYLYSTRPTHR
2   VALLYS

Samples:

sample_1: W4W9 4 ± 0.1 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.02 M; pH: 5.8; pressure: 1 atm; temperature: 281 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

NMRView v5.2.2_01, Johnson, One Moon Scientific - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

AMBER, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollm - refinement, structure solution

NMR spectrometers:

  • Bruker Avance 800 MHz