BMRB Entry 16398

Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR16398

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
1H, 13C and 15N Chemical Shift Assignment for the Psb27 Protein of Synechocystis PCC 6803
Deposition date:
2009-07-08
Original release date:
2009-11-16
Authors:
Cormann, Kai; Nowaczyk, Marc; Ikeuchi, Masahiko; Roegner, Matthias; Stoll, Raphael
Citation:

Citation: Cormann, Kai; Ikeuchi, Masahiko; Rogner, Matthias; Nowaczyk, Marc; Stoll, Raphael. "Sequence-specific (1)H, (13)C, and (15)N backbone assignment of Psb27 from Synechocystis PCC 6803."  Biomol NMR Assign 3, 247-249 (2009).
PubMed: 19789994

Assembly members:

Assembly members:
Psb27, polymer, 110 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Synechocystis sp. PCC 6803   Taxonomy ID: 1148   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Synechocystis sp. PCC 6803

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET3xb (Novagen)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Psb27: CDSGTGLTGNYSQDTLTVIA TLREAIDLPQDAPNRQEVQD TARGQINDYISRYRRKGDAG GLKSFTTMQTALNSLAGYYT SYGARPIPEKLKKRLQLEFT QAERSIERGV

Data sets:
Data typeCount
13C chemical shifts379
15N chemical shifts121
1H chemical shifts614

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Psb271

Entities:

Entity 1, Psb27 110 residues - Formula weight is not available

1   CYSASPSERGLYTHRGLYLEUTHRGLYASN
2   TYRSERGLNASPTHRLEUTHRVALILEALA
3   THRLEUARGGLUALAILEASPLEUPROGLN
4   ASPALAPROASNARGGLNGLUVALGLNASP
5   THRALAARGGLYGLNILEASNASPTYRILE
6   SERARGTYRARGARGLYSGLYASPALAGLY
7   GLYLEULYSSERPHETHRTHRMETGLNTHR
8   ALALEUASNSERLEUALAGLYTYRTYRTHR
9   SERTYRGLYALAARGPROILEPROGLULYS
10   LEULYSLYSARGLEUGLNLEUGLUPHETHR
11   GLNALAGLUARGSERILEGLUARGGLYVAL

Samples:

sample_1: Psb27, [U-15N], 1.0 mM; H2O 90%; D2O 10%; sodium phosphate buffer, pH 6.5 20 mM

sample_2: Psb27, [U-13C; U-15N], 0.9 mM; H2O 90%; D2O 10%; sodium phosphate buffer, pH 6.5 20 mM

sample_conditions_1: ionic strength: 40 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1
3D HNHBsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_1
3D HNCAsample_2isotropicsample_conditions_1
3D CBCA(CO)NHsample_2isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
3D HBHA(CO)NHsample_2isotropicsample_conditions_1
3D HCCH-COSYsample_2isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
3D 1H-13C NOESYsample_2isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - data analysis

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

PDB
DBJ BAA11640 BAA18462 BAK50636 BAL29634 BAL32803
GB AGF52149 ALJ68102
REF WP_041425825
SP P74367
AlphaFold P74367

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks