BMRB Entry 16397
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR16397
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Bonnet, Jacques; Wang, Ying-Hui; Spedale, Gianpiero; Atkinson, R. Andrew; Romier, Christophe; Hamiche, Ali; Pijnappel, W. W M Pim; Timmers, H. Th Marc; Tora, Laszlo; Devys, Didier; Kieffer, Bruno. "The structural plasticity of SCA7 domains defines their differential nucleosome-binding properties." EMBO Rep. 11, 612-618 (2010).
PubMed: 20634802
Assembly members:
SCA7 domain, polymer, 74 residues, 6981.115 Da.
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-4T1
Entity Sequences (FASTA):
SCA7 domain: GSKFLNKRLSEREFDPDIHC
GVIDLDTKKPCTRSLTCKTH
SLTQRRAVQGRRKRFDVLLA
EHKNKTREKELIRH
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 540 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SCA7 domain | 1 |
| 2 | ZINC ION | 2 |
Entities:
Entity 1, SCA7 domain 74 residues - 6981.115 Da.
| 1 | GLY | SER | LYS | PHE | LEU | ASN | LYS | ARG | LEU | SER | ||||
| 2 | GLU | ARG | GLU | PHE | ASP | PRO | ASP | ILE | HIS | CYS | ||||
| 3 | GLY | VAL | ILE | ASP | LEU | ASP | THR | LYS | LYS | PRO | ||||
| 4 | CYS | THR | ARG | SER | LEU | THR | CYS | LYS | THR | HIS | ||||
| 5 | SER | LEU | THR | GLN | ARG | ARG | ALA | VAL | GLN | GLY | ||||
| 6 | ARG | ARG | LYS | ARG | PHE | ASP | VAL | LEU | LEU | ALA | ||||
| 7 | GLU | HIS | LYS | ASN | LYS | THR | ARG | GLU | LYS | GLU | ||||
| 8 | LEU | ILE | ARG | HIS |
Entity 2, ZINC ION - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: sodium phosphate 50 mM; sodium chloride 200 mM; DTT 2 mM; entity_1, [U-99% 13C; U-99% 15N], 0.3 mM; H2O 90%; D2O 10%
sample_2: sodium phosphate 50 mM; sodium chloride 200 mM; DTT 2 mM; entity_1 0.6 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 0.3 M; pH: 7.1; pressure: 1 atm; temperature: 295 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_2 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_2 | isotropic | sample_conditions_1 |
Software:
X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure solution
NMR spectrometers:
- Bruker DRX 600 MHz
- Bruker Avance 950 MHz