BMRB Entry 16386

Name: Solution NMR Structure of the EGF-like 1 Domain of Human Fibulin-4. Northeast Structural Genomics Target HR6275.
Published: 2009-08-06

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PDB ID: 2kl7
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR16386
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution NMR Structure of the EGF-like 1 Domain of Human Fibulin-4. Northeast Structural Genomics Target HR6275.

Deposition date:

2009-06-30

Original release date:

2009-08-06

Authors:

Rossi, Paolo; Chiang, Yiwen; Anderson, Stephen; Montelione, Gaetano

Citation:

Citation: Rossi, Paolo; Chiang, Yiwen; Anderson, Stephen; Montelione, Gaetano. "Solution NMR Structure of the EGF-like 1 Domain of Human Fibulin-4. Northeast Structural Genomics Target HR6275." .

Assembly members:

Assembly members:
EGF-like 1 Domain, polymer, 71 residues, 7576.472 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: na

Entity Sequences (FASTA):

Entity Sequences (FASTA):
EGF-like 1 Domain: SDVNECLTIPEACKGEMKCI NHYGGYLCLPRSAAVINDLH GEGPPPPVPPAQHPNPCPPG YEPDDQDSCVD

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	264
15N chemical shifts	67
1H chemical shifts	407

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	EGF-like 1 Domain	1

Entities:

Entity 1, EGF-like 1 Domain 71 residues - 7576.472 Da.

1

SER

ASP

VAL

ASN

GLU

CYS

LEU

THR

ILE

PRO

2

GLU

ALA

CYS

LYS

GLY

GLU

MET

LYS

CYS

ILE

3

ASN

HIS

TYR

GLY

TYR

LEU

CYS

LEU

PRO

4

ARG

SER

ALA

VAL

ILE

ASN

ASP

LEU

HIS

5

GLY

GLU

GLY

PRO

VAL

PRO

6

ALA

GLN

HIS

PRO

ASN

PRO

CYS

PRO

GLY

7

TYR

GLU

PRO

ASP

GLN

ASP

SER

CYS

VAL

8

ASP

Samples:

sample_1: entity, [U-100% 13C; U-100% 15N], 0.6 mM; sodium chloride 150 mM; MES 20 mM; DSS 50 uM; H2O 90%; D2O 10%

sample_2: entity, [U-100% 15N], 0.3 mM; sodium chloride 150 mM; MES 20 mM; DSS 50 uM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.15 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D HCCH-COSY	sample_1	isotropic	sample_conditions_1
3D CCH-TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-15N HMQC	sample_1	isotropic	sample_conditions_1
2D 1H-15N het_NOE	sample_1	isotropic	sample_conditions_1
1D 15N_T1 series	sample_1	isotropic	sample_conditions_1
1D 15N_T2 series	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions_1

Software:

CYANA v3.0, Guntert, Mumenthaler and Wuthrich - structure solution

CNS v1.3, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

TOPSPIN v2.1, Bruker Biospin - collection

PSVS v1.3, Bhattacharya and Montelione - validation

SPARKY v2.113, Goddard - data analysis

PINE, Bahrami, Markley, Assadi, and Eghbalnia - data analysis

Molmol, Koradi, Billeter and Wuthrich - visualization

PyMol, DeLano Scientific - visualization

PDBStat v5.1, Tejero, Montelione - PDB processing

NMR spectrometers:

Bruker Avance 800 MHz
Bruker Avance 600 MHz

PDB	2KL7
DBJ	BAA92880 BAD92358 BAF84768 BAG37622 BAG50843
EMBL	CAA10791 CAG46732
GB	AAC03101 AAF65188 AAG45245 AAH10456 AAQ89258
REF	NP_001231778 NP_058634 XP_001118071 XP_003274253 XP_003909598
SP	O55058 O95967
AlphaFold	O55058 O95967