BMRB Entry 16385

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PDB ID: 2kl6
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR16385
MolProbity Validation Chart

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Title:
Solution NMR structure of the CARDB domain of PF1109 from Pyrococcus furiosus. Northeast Structural Genomics Consortium target PfR193A
Deposition date:
2009-06-30
Original release date:
2009-07-06
Authors:
Aramini, James; Lee, Dong-Yup; Ciccosanti, Colleen; Hamilton, Keith; Nair, Rajesh; Rost, Burkhard; Acton, Thomas; Xiao, Rong; Swapna, Gurla; Everett, John; Montelione, Gaetano
Citation:

Citation: Aramini, James; Lee, Dong-Yup; Ciccosanti, Colleen; Hamilton, Keith; Nair, Rajesh; Rost, Burkhard; Acton, Thomas; Xiao, Rong; Swapna, Gurla; Everett, John; Montelione, Gaetano. "Solution NMR structure of the CARDB domain of PF1109 from Pyrococcus furiosus."  .

Assembly members:

Assembly members:
CARDB domain, polymer, 114 residues, 12868.545 Da.

Natural source:

Natural source:   Common Name: Pyrococcus furiosus   Taxonomy ID: 2261   Superkingdom: Archaea   Kingdom: not available   Genus/species: Pyrococcus furiosus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: PfR193A-436-540-21.36

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CARDB domain: MEFPDLTVEIKGPDVVGVNK LAEYEVHVKNLGGIGVPSTK VRVYINGTLYKNWTVSLGPK EEKVLTFSWTPTQEGMYRIN ATVDEENTVVELNENNNVAT FDVSVVLEHHHHHH

Data sets:
Data typeCount
13C chemical shifts498
15N chemical shifts114
1H chemical shifts788

Time Domain Data

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1CARDB domain1

Entities:

Entity 1, CARDB domain 114 residues - 12868.545 Da.

Contains additional N-terminal Met and C-terminal LEHHHHHH affinity tag due to cloning.

1   METGLUPHEPROASPLEUTHRVALGLUILE
2   LYSGLYPROASPVALVALGLYVALASNLYS
3   LEUALAGLUTYRGLUVALHISVALLYSASN
4   LEUGLYGLYILEGLYVALPROSERTHRLYS
5   VALARGVALTYRILEASNGLYTHRLEUTYR
6   LYSASNTRPTHRVALSERLEUGLYPROLYS
7   GLUGLULYSVALLEUTHRPHESERTRPTHR
8   PROTHRGLNGLUGLYMETTYRARGILEASN
9   ALATHRVALASPGLUGLUASNTHRVALVAL
10   GLULEUASNGLUASNASNASNVALALATHR
11   PHEASPVALSERVALVALLEUGLUHISHIS
12   HISHISHISHIS

Related Database Links:

PDB 2KL6 2M8X 3IDU
GB AAL81233 AFN03901
REF WP_014835329

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks