BMRB Entry 16374

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PDB ID: 2kkx
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR16374
MolProbity Validation Chart

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Title:
Solution Structure of C-terminal domain of reduced NleG2-3 (residue 90-191) from Pathogenic E. coli O157:H7. Northeast Structural Genomics Consortium and Midwest Center for Structural Genomics target ET109A
Deposition date:
2009-06-29
Original release date:
2009-09-15
Authors:
Wu, Bin; Yee, Adelinda; Fares, Christophe; Lemak, Alexander; Semest, Anthony; Claude, Marie; Singer, Alexander; Edwards, Aled; Savchenko, Alexei; Arrowsmith, Cheryl
Citation:

Citation: Wu, Bin; Yee, Adelinda; Fares, Christophe; Lemak, Alexander; Semest, Anthony; Claude, Marie; Coomes, Brian; Edwards, Aled; Singer, Alexander; Arrowsmith, Cheryl; Savchenko, Alexei. "Pathogenic E. coli O157:H7 NleG family of Type 3 secretion effectors features a common C-terminal U-box domain and E3 ubiquitin ligase activity"  .

Assembly members:

Assembly members:
NleG2-3, polymer, 102 residues, 11290.936 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
NleG2-3: SQESIQNKISQCKFSVCPER LQCPLEAIQCPITLEQPEKG IFVKNSDGSDVCTLFDAAAF SRLVGEGLPHPLTREPITAS IIVKHEECIYDDTRGNFIIK GN

Data typeCount
13C chemical shifts432
15N chemical shifts104
1H chemical shifts721
residual dipolar couplings186

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Assembly:

Entity Assembly IDEntity NameEntity ID
1NleG2-31

Entities:

Entity 1, NleG2-3 102 residues - 11290.936 Da.

1   SERGLNGLUSERILEGLNASNLYSILESER
2   GLNCYSLYSPHESERVALCYSPROGLUARG
3   LEUGLNCYSPROLEUGLUALAILEGLNCYS
4   PROILETHRLEUGLUGLNPROGLULYSGLY
5   ILEPHEVALLYSASNSERASPGLYSERASP
6   VALCYSTHRLEUPHEASPALAALAALAPHE
7   SERARGLEUVALGLYGLUGLYLEUPROHIS
8   PROLEUTHRARGGLUPROILETHRALASER
9   ILEILEVALLYSHISGLUGLUCYSILETYR
10   ASPASPTHRARGGLYASNPHEILEILELYS
11   GLYASN

Related Database Links:

BMRB 16375
PDB 2KKX 2KKY
DBJ BAB35417 BAB35579 BAI25254 BAI35676
EMBL CTT86631 CTZ63346 CTZ65248 CTZ72681 CTZ98837
GB AAG56215 AAG56388 ACI35420 ACT71854 ADD56326
REF NP_310021 NP_310183 WP_001131656 WP_001131657 WP_001131658

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