BMRB Entry 16363

Name: Solution structure of the Nuclear coactivator binding domain of CBP
Published: 2010-08-16

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PDB ID: 2kkj
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16363
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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the Nuclear coactivator binding domain of CBP

Deposition date:

2009-06-24

Original release date:

2010-08-16

Authors:

Kjaergaard, Magnus; Teilum, Kaare; Poulsen, Flemming

Citation:

Citation: Kjaergaard, Magnus; Teilum, Kaare; Poulsen, Flemming. "Conformational selection in the molten globule state of the nuclear coactivator binding domain of CBP" Proc. Natl. Acad. Sci. U S A. 107, 12535-12540 (2010).
PubMed: 20616042

Assembly members:

Assembly members:
CBP, polymer, 59 residues, 6568.623 Da.

Natural source:

Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET22b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CBP: PNRSISPSALQDLLRTLKSP SSPQQQQQVLNILKSNPQLM AAFIKQRTAKYVANQPGMQ

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	236
15N chemical shifts	49
1H chemical shifts	372

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	CBP	1

Entities:

Entity 1, CBP 59 residues - 6568.623 Da.

1

PRO

ASN

ARG

SER

ILE

SER

PRO

SER

ALA

LEU

2

GLN

ASP

LEU

ARG

THR

LEU

LYS

SER

PRO

3

SER

PRO

GLN

VAL

LEU

4

ASN

ILE

LEU

LYS

SER

ASN

PRO

GLN

LEU

MET

5

ALA

PHE

ILE

LYS

GLN

ARG

THR

ALA

LYS

6

TYR

VAL

ALA

ASN

GLN

PRO

GLY

MET

GLN

Samples:

sample_1: D2O 10%; sodium chloride 20 mM; sodium phosphate 20 mM; protein, [U-100% 13C; U-100% 15N], 2 mM; H2O 90%

sample_conditions_1: ionic strength: 0.1 M; pH: 6.5; pressure: 1 atm; temperature: 304 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1

Software:

ARIA v2.2, Linge, O'Donoghue and Nilges - structure solution

NMR spectrometers:

Varian INOVA 750 MHz
Bruker Avance 800 MHz

BMRB	15398 17073
PDB	1JJS 1KBH 1ZOQ 2C52 2KKJ 2L14
DBJ	BAE06125 BAI45616
GB	AAB28651 AAC17736 AAC51331 AAC51340 AAC51770
PRF	1923401A
REF	NP_001020603 NP_001073315 NP_001247644 NP_004371 NP_596872
SP	P45481 Q6JHU9 Q92793
AlphaFold	P45481 Q6JHU9 Q92793