BMRB Entry 16359

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR16359

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Title:
chemical shift assignment of West Nile protease in the absence of inhibitor
Deposition date:
2009-06-19
Original release date:
2009-12-16
Authors:
Su, Xun-Cheng
Citation:

Citation: Su, Xun-Cheng; Ozawa, Kiyoshi; Qi, Ruhu; Vasudevan, Subhash; Lim, Siew; Otting, Gottfried. "NMR analysis of the dynamic exchange of the NS2B cofactor between open and closed conformations of the West Nile virus NS2B-NS3 protease."  PLoS Negl. Trop. Dis. 3, .-. (2009).
PubMed: 19997625

Assembly members:

Assembly members:
West_Nile_virus_protease, polymer, 226 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: west nile virus   Taxonomy ID: 11082   Superkingdom: virus   Kingdom: not available   Genus/species: Flavivirus west nile virus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15b-WNV CF40GlyNS3pro187

Entity Sequences (FASTA):

Entity Sequences (FASTA):
West_Nile_virus_protease: GDTTTGVYRIMTRGLLGSYQ AGAGVMVEGVFHTLWHTTKG AALMSGEGRLDPYWGSVKED RLCYGGPWKLQHKWNGHDEV QMIVVEPGKNVKNVQTKPGV FKTPEGEIGAVTLDYPTGTS GSPIVDKNGDVIGLYGNGVI MPNGSYISAIVQGERMEEPA PAGFEPEMLRKKGSHMLETD MWIERTADITWESDAEITGS SERVDVRLDDDGNFQLMNDP GAPWAG

Data sets:
  • assigned_chemical_shifts
Data typeCount
15N chemical shifts200
1H chemical shifts200

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1NS2B_NS3pro1

Entities:

Entity 1, NS2B_NS3pro 226 residues - Formula weight is not available

1   GLYASPTHRTHRTHRGLYVALTYRARGILE
2   METTHRARGGLYLEULEUGLYSERTYRGLN
3   ALAGLYALAGLYVALMETVALGLUGLYVAL
4   PHEHISTHRLEUTRPHISTHRTHRLYSGLY
5   ALAALALEUMETSERGLYGLUGLYARGLEU
6   ASPPROTYRTRPGLYSERVALLYSGLUASP
7   ARGLEUCYSTYRGLYGLYPROTRPLYSLEU
8   GLNHISLYSTRPASNGLYHISASPGLUVAL
9   GLNMETILEVALVALGLUPROGLYLYSASN
10   VALLYSASNVALGLNTHRLYSPROGLYVAL
11   PHELYSTHRPROGLUGLYGLUILEGLYALA
12   VALTHRLEUASPTYRPROTHRGLYTHRSER
13   GLYSERPROILEVALASPLYSASNGLYASP
14   VALILEGLYLEUTYRGLYASNGLYVALILE
15   METPROASNGLYSERTYRILESERALAILE
16   VALGLNGLYGLUARGMETGLUGLUPROALA
17   PROALAGLYPHEGLUPROGLUMETLEUARG
18   LYSLYSGLYSERHISMETLEUGLUTHRASP
19   METTRPILEGLUARGTHRALAASPILETHR
20   TRPGLUSERASPALAGLUILETHRGLYSER
21   SERGLUARGVALASPVALARGLEUASPASP
22   ASPGLYASNPHEGLNLEUMETASNASPPRO
23   GLYALAPROTRPALAGLY

Related Database Links:

PDB 2FP7
GB AEL79849 AFX82689 AGC00412
REF NP_776018

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CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks