BMRB Entry 16321

Name: Backbone 1H, 13C and 15N resonance assignments for the E2 conjugating enzyme, UbcH8
Published: 2009-10-14

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PDB ID: 2kjh
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16321
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C and 15N resonance assignments for the E2 conjugating enzyme, UbcH8

Deposition date:

2009-05-28

Original release date:

2009-10-14

Authors:

Serniwka, Stephanie; Shaw, Gary

Citation:

Citation: Serniwka, Stephanie; Shaw, Gary. "The structure of the UbcH8-ubiquitin complex shows a unique ubiquitin interaction site" Biochemistry 48, 12169-12179 (2009).
PubMed: 19928833

Assembly members:

Assembly members:
UbcH8, polymer, 152 residues, 17638 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: p11

Entity Sequences (FASTA):

Entity Sequences (FASTA):
UbcH8: MASMRVVKELEDLQKKPPPY LRNLSSDDANVLVWHALLLP DQPPYHLKAFNLRISFPPEY PFKPPMIKFTTKIYHPNVDE NGQICLPIISSENWKPSTKT SQVLEALNVLVNRPNIREPL RMDLADLLTQNPELFRKNAE EFTLRFGVDRPS

Data sets:

assigned_chemical_shifts
- Assigned_Chemical_Shifts_for_UbcH8

Data type	Count
13C chemical shifts	460
15N chemical shifts	140
1H chemical shifts	446

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	UbcH8	1

Entities:

Entity 1, UbcH8 152 residues - 17638 Da.

1

MET

ALA

SER

MET

ARG

VAL

LYS

GLU

LEU

2

GLU

ASP

LEU

GLN

LYS

PRO

TYR

3

LEU

ARG

ASN

LEU

SER

ASP

ALA

ASN

4

VAL

LEU

VAL

TRP

HIS

ALA

LEU

PRO

5

ASP

GLN

PRO

TYR

HIS

LEU

LYS

ALA

PHE

6

ASN

LEU

ARG

ILE

SER

PHE

PRO

GLU

TYR

7

PRO

PHE

LYS

PRO

MET

ILE

LYS

PHE

THR

8

THR

LYS

ILE

TYR

HIS

PRO

ASN

VAL

ASP

GLU

9

ASN

GLY

GLN

ILE

CYS

LEU

PRO

ILE

SER

10

SER

GLU

ASN

TRP

LYS

PRO

SER

THR

LYS

THR

11

SER

GLN

VAL

LEU

GLU

ALA

LEU

ASN

VAL

LEU

12

VAL

ASN

ARG

PRO

ASN

ILE

ARG

GLU

PRO

LEU

13

ARG

MET

ASP

LEU

ALA

ASP

LEU

THR

GLN

14

ASN

PRO

GLU

LEU

PHE

ARG

LYS

ASN

ALA

GLU

15

GLU

PHE

THR

LEU

ARG

PHE

GLY

VAL

ASP

ARG

16

PRO

SER

Samples:

sample_UbcH8: UbcH8, [U-100% 13C; U-100% 15N], 0.5 mM; sodium phosphate 20 mM; EDTA 1 mM; DTT 1 mM; sodium chloride 150 mM; Arginine 50 mM; Glutamic Acid 50 mM; H2O 90%; D2O 10%

sample_conditions_for_UbcH8: ionic strength: 150 mM; pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_UbcH8	isotropic	sample_conditions_for_UbcH8
3D CBCA(CO)NH	sample_UbcH8	isotropic	sample_conditions_for_UbcH8
3D HNCACB	sample_UbcH8	isotropic	sample_conditions_for_UbcH8
3D HNCO	sample_UbcH8	isotropic	sample_conditions_for_UbcH8
3D C(CO)NH	sample_UbcH8	isotropic	sample_conditions_for_UbcH8
3D H(CCO)NH	sample_UbcH8	isotropic	sample_conditions_for_UbcH8

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

VNMRJ v2.1B, Varian - collection

NMR spectrometers:

Varian INOVA 600 MHz

SWS	O14933
PDB	1WZV 1WZW 2KJH
DBJ	BAG38109
EMBL	CAB64566 CAG33407
GB	AAB86433 AAD17525 AAH32491 ABM82217 ABM85401
REF	NP_004214 NP_937826 XP_003318029 XP_003826475 XP_004051215
SP	O14933
AlphaFold	O14933