BMRB Entry 16319

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR16319
MolProbity Validation Chart

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Title:
The solution structure of the circular bacteriocin carnocyclin A (CclA)
Deposition date:
2009-05-28
Original release date:
2009-09-04
Authors:
Martin-Visscher, Leah; Gong, Xiandi; Duszyk, Marek; Vederas, John
Citation:

Citation: Martin-Visscher, Leah; Gong, Xiandi; Duszyk, Marek; Vederas, John. "The three-dimensional structure of carnocyclin A reveals that many circular bacteriocins share a common structural motif."  J. Biol. Chem. 284, 28674-28681 (2009).
PubMed: 19692336

Assembly members:

Assembly members:
Carnocyclin_A, polymer, 60 residues, 5864 Da.

Natural source:

Natural source:   Common Name: Carnobacterium maltaromaticum   Taxonomy ID: 2751   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Carnobacterium maltaromaticum

Experimental source:

Experimental source:   Production method: purified from the natural source   Host organism: Carnobacterium maltaromaticum (Carnobacterium piscicola)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Carnocyclin_A: LVAYGIAQGTAEKVVSLINA GLTVGSIISILGGVTVGLSG VFTAVKAAIAKQGIKKAIQL

Data sets:
Data typeCount
13C chemical shifts261
15N chemical shifts64
1H chemical shifts441

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Carnocyclin_A1

Entities:

Entity 1, Carnocyclin_A 60 residues - 5864 Da.

Covalent bond between N- and C- termini

1   LEUVALALATYRGLYILEALAGLNGLYTHR
2   ALAGLULYSVALVALSERLEUILEASNALA
3   GLYLEUTHRVALGLYSERILEILESERILE
4   LEUGLYGLYVALTHRVALGLYLEUSERGLY
5   VALPHETHRALAVALLYSALAALAILEALA
6   LYSGLNGLYILELYSLYSALAILEGLNLEU

Samples:

Sample_1: Carnocyclin A, [U-99% 13C; U-99% 15N], 1 mM; sodium phosphate 20 mM; EDTA 1 mM; sodium azide 1 mM; DSS 0.1 mM; H2O 90%; D2O 10%

Sample_2: Carnocyclin A, [U-99% 13C; U-99% 15N], 1 mM; sodium phosphate 20 mM; EDTA 1 mM; sodium azide 1 mM; DSS 0.1 mM; D2O 100%

Condition_1: pH: 5.9; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCSample_1isotropicCondition_1
3D HNCOSample_1isotropicCondition_1
3D HNCACBSample_1isotropicCondition_1
3D CBCA(CO)NHSample_1isotropicCondition_1
3D 1H-15N NOESYSample_1isotropicCondition_1
3D 1H-15N TOCSYSample_1isotropicCondition_1
3D HNHASample_1isotropicCondition_1
2D 1H-1H TOCSYSample_2isotropicCondition_1
2D 1H-1H COSYSample_2isotropicCondition_1
2D 1H-1H NOESYSample_2isotropicCondition_1
2D 1H-13C HSQCSample_2isotropicCondition_1
3D HCCH-TOCSYSample_2isotropicCondition_1
3D 1H-13C NOESYSample_2isotropicCondition_1

Software:

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - refinement, structure solution

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
GB ACC93994
SP B2MVM5
AlphaFold B2MVM5

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks