BMRB Entry 16318
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR16318
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Ferreon, Josephine; Martinez-Yamout, Maria; Dyson, H. Jane; Wright, Peter. "Structural Basis for Subversion of Cellular Control Mechanisms by the Adenoviral E1A Oncoprotein" Proc. Natl. Acad. Sci. U.S.A. 106, 13260-13265 (2009).
PubMed: 19651603
Assembly members:
CBP_TAZ2, polymer, 92 residues, 10527.605 Da.
E1A, polymer, 42 residues, 4464.949 Da.
ZN, non-polymer, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET21a
Entity Sequences (FASTA):
CBP_TAZ2: SPQESRRLSIQRCIQSLVHA
CQCRNANCSLPSCQKMKRVV
QHTKGCKRKTNGGCPVCKQL
IALCCYHAKHCQENKCPVPF
CLNIKHKLRQQQ
E1A: SHMAPEDPNEEAVSQIFPDS
VMLAVQEGIDLLTFPPAPGS
PE
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 424 |
| 15N chemical shifts | 131 |
| 1H chemical shifts | 917 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | CBP_TAZ2 | 1 |
| 2 | E1A | 2 |
| 3 | ZINC ION_1 | 3 |
| 4 | ZINC ION_2 | 3 |
| 5 | ZINC ION_3 | 3 |
Entities:
Entity 1, CBP_TAZ2 92 residues - 10527.605 Da.
| 1 | SER | PRO | GLN | GLU | SER | ARG | ARG | LEU | SER | ILE | ||||
| 2 | GLN | ARG | CYS | ILE | GLN | SER | LEU | VAL | HIS | ALA | ||||
| 3 | CYS | GLN | CYS | ARG | ASN | ALA | ASN | CYS | SER | LEU | ||||
| 4 | PRO | SER | CYS | GLN | LYS | MET | LYS | ARG | VAL | VAL | ||||
| 5 | GLN | HIS | THR | LYS | GLY | CYS | LYS | ARG | LYS | THR | ||||
| 6 | ASN | GLY | GLY | CYS | PRO | VAL | CYS | LYS | GLN | LEU | ||||
| 7 | ILE | ALA | LEU | CYS | CYS | TYR | HIS | ALA | LYS | HIS | ||||
| 8 | CYS | GLN | GLU | ASN | LYS | CYS | PRO | VAL | PRO | PHE | ||||
| 9 | CYS | LEU | ASN | ILE | LYS | HIS | LYS | LEU | ARG | GLN | ||||
| 10 | GLN | GLN |
Entity 2, E1A 42 residues - 4464.949 Da.
| 1 | SER | HIS | MET | ALA | PRO | GLU | ASP | PRO | ASN | GLU | ||||
| 2 | GLU | ALA | VAL | SER | GLN | ILE | PHE | PRO | ASP | SER | ||||
| 3 | VAL | MET | LEU | ALA | VAL | GLN | GLU | GLY | ILE | ASP | ||||
| 4 | LEU | LEU | THR | PHE | PRO | PRO | ALA | PRO | GLY | SER | ||||
| 5 | PRO | GLU |
Entity 3, ZINC ION_1 - Formula weight is not available
| 1 | ZN |
Samples:
E1A:TAZ2: entity_1, [U-100% 13C; U-100% 15N], 1 – 1.5 mM; entity_2, [U-100% 15N], 1 – 1.5 mM; D2O 100%
E1A:TAZ2_2: entity_1, [U-100% 13C; U-100% 15N], 1 – 1.5 mM; entity_2, [U-100% 15N], 1 – 1.5 mM; Tris 20 mM; NaCl 50 mM; D2O 100%
10_mM_dTris_pH_6.8: ionic strength: 0 M; pH: 6.8; pressure: 1 atm; temperature: 298 K
20_mM_Tris_50mM_NaCl_pH_6.8: ionic strength: 70 mM; pH: 6.8; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | E1A:TAZ2_2 | isotropic | 20_mM_Tris_50mM_NaCl_pH_6.8 |
| 3D HNCA | E1A:TAZ2 | isotropic | 10_mM_dTris_pH_6.8 |
Software:
AMBER, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollm - data analysis
NMR spectrometers:
- Bruker DRX 600 MHz
- Bruker DRX 800 MHz
- Bruker Avance 900 MHz
Related Database Links:
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks