BMRB Entry 16303

Name: SLAS-bound SaS synuclein variant
Published: 2009-06-03

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16303

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

SLAS-bound SaS synuclein variant

Deposition date:

2009-05-14

Original release date:

2009-06-03

Authors:

Rao, Jampani Nageswara; Kim, Yujin; Park, Leena; Ulmer, Tobias

Citation:

Citation: Rao, Jampani Nageswara; Kim, Yujin; Park, Leena; Ulmer, Tobias. "Effect of Pseudorepeat Rearrangement on alpha-Synuclein Misfolding, Vesicle Binding, and Micelle Binding" J. Mol. Biol. 390, 516-529 (2009).
PubMed: 19481090

Assembly members:

Assembly members:
SLAS-bound_SaS, polymer, 140 residues, Formula weight is not available

Natural source:

Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not applicable

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET44-SaS

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SLAS-bound_SaS: MDVFMKGLEKTKEQVTNVGS KTKEGVVHGVGAVVTGVTAV AEKTKQGVAEAAATVASKAK EGVVAAAGKTKEGVLYVGQK TVEGAGSIAAATGFVKKDQL GKNEEGAPQEGILEDMPVDP DNEAYEMPSEEGYQDYEPEA

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	380
15N chemical shifts	133
1H chemical shifts	133

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	monomer	1

Entities:

Entity 1, monomer 140 residues - Formula weight is not available

1	MET	ASP	VAL	PHE	MET	LYS	GLY	LEU	GLU	LYS
2	THR	LYS	GLU	GLN	VAL	THR	ASN	VAL	GLY	SER
3	LYS	THR	LYS	GLU	GLY	VAL	VAL	HIS	GLY	VAL
4	GLY	ALA	VAL	VAL	THR	GLY	VAL	THR	ALA	VAL
5	ALA	GLU	LYS	THR	LYS	GLN	GLY	VAL	ALA	GLU
6	ALA	ALA	ALA	THR	VAL	ALA	SER	LYS	ALA	LYS
7	GLU	GLY	VAL	VAL	ALA	ALA	ALA	GLY	LYS	THR
8	LYS	GLU	GLY	VAL	LEU	TYR	VAL	GLY	GLN	LYS
9	THR	VAL	GLU	GLY	ALA	GLY	SER	ILE	ALA	ALA
10	ALA	THR	GLY	PHE	VAL	LYS	LYS	ASP	GLN	LEU
11	GLY	LYS	ASN	GLU	GLU	GLY	ALA	PRO	GLN	GLU
12	GLY	ILE	LEU	GLU	ASP	MET	PRO	VAL	ASP	PRO
13	ASP	ASN	GLU	ALA	TYR	GLU	MET	PRO	SER	GLU
14	GLU	GLY	TYR	GLN	ASP	TYR	GLU	PRO	GLU	ALA

Samples:

sample_1: SaS, [U-100% 13C; U-100% 15N; U-80% 2H], 0.75 mM; sodium lauroyl sarcosinate 75 mM; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 0.05 M; pH: 7.4; pressure: 1 atm; temperature: 298.2 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1

Software:

CARA, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

Bruker Avance 700 MHz

1	MET	ASP	VAL	PHE	MET	LYS	GLY	LEU	GLU	LYS
2	THR	LYS	GLU	GLN	VAL	THR	ASN	VAL	GLY	SER
3	LYS	THR	LYS	GLU	GLY	VAL	VAL	HIS	GLY	VAL
4	GLY	ALA	VAL	VAL	THR	GLY	VAL	THR	ALA	VAL
5	ALA	GLU	LYS	THR	LYS	GLN	GLY	VAL	ALA	GLU
6	ALA	ALA	ALA	THR	VAL	ALA	SER	LYS	ALA	LYS
7	GLU	GLY	VAL	VAL	ALA	ALA	ALA	GLY	LYS	THR
8	LYS	GLU	GLY	VAL	LEU	TYR	VAL	GLY	GLN	LYS
9	THR	VAL	GLU	GLY	ALA	GLY	SER	ILE	ALA	ALA
10	ALA	THR	GLY	PHE	VAL	LYS	LYS	ASP	GLN	LEU
11	GLY	LYS	ASN	GLU	GLU	GLY	ALA	PRO	GLN	GLU
12	GLY	ILE	LEU	GLU	ASP	MET	PRO	VAL	ASP	PRO
13	ASP	ASN	GLU	ALA	TYR	GLU	MET	PRO	SER	GLU
14	GLU	GLY	TYR	GLN	ASP	TYR	GLU	PRO	GLU	ALA

1	MET	ASP	VAL	PHE	MET	LYS	GLY	LEU	GLU	LYS
2	THR	LYS	GLU	GLN	VAL	THR	ASN	VAL	GLY	SER
3	LYS	THR	LYS	GLU	GLY	VAL	VAL	HIS	GLY	VAL
4	GLY	ALA	VAL	VAL	THR	GLY	VAL	THR	ALA	VAL
5	ALA	GLU	LYS	THR	LYS	GLN	GLY	VAL	ALA	GLU
6	ALA	ALA	ALA	THR	VAL	ALA	SER	LYS	ALA	LYS
7	GLU	GLY	VAL	VAL	ALA	ALA	ALA	GLY	LYS	THR
8	LYS	GLU	GLY	VAL	LEU	TYR	VAL	GLY	GLN	LYS
9	THR	VAL	GLU	GLY	ALA	GLY	SER	ILE	ALA	ALA
10	ALA	THR	GLY	PHE	VAL	LYS	LYS	ASP	GLN	LEU
11	GLY	LYS	ASN	GLU	GLU	GLY	ALA	PRO	GLN	GLU
12	GLY	ILE	LEU	GLU	ASP	MET	PRO	VAL	ASP	PRO
13	ASP	ASN	GLU	ALA	TYR	GLU	MET	PRO	SER	GLU
14	GLU	GLY	TYR	GLN	ASP	TYR	GLU	PRO	GLU	ALA