BMRB Entry 16299

Name: NMR assignment of a 48 kDa tetramer of the T1 domain of the mammalian voltage gated potassium channel Kv1.4
Published: 2009-07-22

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR16299

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR assignment of a 48 kDa tetramer of the T1 domain of the mammalian voltage gated potassium channel Kv1.4

Deposition date:

2009-05-13

Original release date:

2009-07-22

Authors:

Schreier, Christina; Auer, Alexandra; Kalbitzer, Hans Robert; Kremer, Werner

Citation:

Citation: Schreier, Christina; Auer, Alexandra; Kalbitzer, Hans Robert; Kremer, Werner. "NMR assignments of a 48 kDa tetramer of the T1 domain of the mammalian voltage gated potassium channel Kv1.4." Biomol NMR Assign 3, 167-170 (2009).
PubMed: 19888682

Assembly members:

Assembly members:
T1_domain_of_Kv1.4, polymer, 101 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET20b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
T1_domain_of_Kv1.4: MERVVINVSGLRFETQMKTL AQFPETLLGDPEKRTQYFDP LRNEYFFDRNRPSFDAILYY YQSGGRLKRPVNVPFDIFTE EVKFYQLGKLAAALEHHHHH H

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	244
15N chemical shifts	90
1H chemical shifts	90

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	T1 domain	1

Entities:

Entity 1, T1 domain 101 residues - Formula weight is not available

1

MET

GLU

ARG

VAL

ILE

ASN

VAL

SER

GLY

2

LEU

ARG

PHE

GLU

THR

GLN

MET

LYS

THR

LEU

3

ALA

GLN

PHE

PRO

GLU

THR

LEU

GLY

ASP

4

PRO

GLU

LYS

ARG

THR

GLN

TYR

PHE

ASP

PRO

5

LEU

ARG

ASN

GLU

TYR

PHE

ASP

ARG

ASN

6

ARG

PRO

SER

PHE

ASP

ALA

ILE

LEU

TYR

7

TYR

GLN

SER

GLY

ARG

LEU

LYS

ARG

PRO

8

VAL

ASN

VAL

PRO

PHE

ASP

ILE

PHE

THR

GLU

9

GLU

VAL

LYS

PHE

TYR

GLN

LEU

GLY

LYS

LEU

10

ALA

LEU

GLU

HIS

11

HIS

Samples:

sample_1: T1 domain of Kv1.4, [U-13C; U-15N; U-2H], 1 mM; H2O 93%; D2O 7%

sample_conditions_1: ionic strength: 150 mM; pH: 6.5; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CACB	sample_1	isotropic	sample_conditions_1
3D HN(CA)NNH	sample_1	isotropic	sample_conditions_1

Software:

AUREMOL, Bruker Biospin - data analysis

NMR spectrometers:

Bruker Avance 800 MHz
Bruker Avance 600 MHz

GB	EHB17201 ELR56484
REF	XP_003773706 XP_007497268 XP_007657298 XP_007657303 XP_007657307