BMRB Entry 16299

Title:
NMR assignment of a 48 kDa tetramer of the T1 domain of the mammalian voltage gated potassium channel Kv1.4
Deposition date:
2009-05-13
Original release date:
2009-07-22
Authors:
Schreier, Christina; Auer, Alexandra; Kalbitzer, Hans Robert; Kremer, Werner
Citation:

Citation: Schreier, Christina; Auer, Alexandra; Kalbitzer, Hans Robert; Kremer, Werner. "NMR assignments of a 48 kDa tetramer of the T1 domain of the mammalian voltage gated potassium channel Kv1.4."  Biomol NMR Assign 3, 167-170 (2009).
PubMed: 19888682

Assembly members:

Assembly members:
T1_domain_of_Kv1.4, polymer, 101 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET20b

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts244
15N chemical shifts90
1H chemical shifts90

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1T1 domain1

Entities:

Entity 1, T1 domain 101 residues - Formula weight is not available

1   METGLUARGVALVALILEASNVALSERGLY
2   LEUARGPHEGLUTHRGLNMETLYSTHRLEU
3   ALAGLNPHEPROGLUTHRLEULEUGLYASP
4   PROGLULYSARGTHRGLNTYRPHEASPPRO
5   LEUARGASNGLUTYRPHEPHEASPARGASN
6   ARGPROSERPHEASPALAILELEUTYRTYR
7   TYRGLNSERGLYGLYARGLEULYSARGPRO
8   VALASNVALPROPHEASPILEPHETHRGLU
9   GLUVALLYSPHETYRGLNLEUGLYLYSLEU
10   ALAALAALALEUGLUHISHISHISHISHIS
11   HIS

Samples:

sample_1: T1 domain of Kv1.4, [U-13C; U-15N; U-2H], 1 mM; H2O 93%; D2O 7%

sample_conditions_1: ionic strength: 150 mM; pH: 6.5; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HN(CO)CACBsample_1isotropicsample_conditions_1
3D HN(CA)NNHsample_1isotropicsample_conditions_1

Software:

AUREMOL, Bruker Biospin - data analysis

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker Avance 600 MHz

Related Database Links:

GB EHB17201 ELR56484
REF XP_003773706 XP_007497268 XP_007657298 XP_007657303 XP_007657307

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks