BMRB Entry 16296

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16296

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Title:
NMR Assignment of the Intrinsically Disordered C-terminal Region of Homo sapiens FCP1 in the Unbound State
Deposition date:
2009-05-12
Original release date:
2009-06-11
Authors:
Showalter, Scott
Citation:

Citation: Showalter, Scott. "NMR assignment of the intrinsically disordered C-terminal region of Homo sapiens FCP1 in the unbound state."  Biomol. NMR Assignments 3, 179-181 (2009).
PubMed: 19888685

Assembly members:

Assembly members:
FCP1, polymer, 85 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-47b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
FCP1: PGPEEQEEEPQPRKPGTRRE RTLGAPASSERSAAGGRGPR GHKRKLNEEDAASESSRESS NEDEGSSSEADEMAKALEAE LNDLM

Data sets:
Data typeCount
13C chemical shifts321
15N chemical shifts86
1H chemical shifts422

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1FCP11

Entities:

Entity 1, FCP1 85 residues - Formula weight is not available

1   PROGLYPROGLUGLUGLNGLUGLUGLUPRO
2   GLNPROARGLYSPROGLYTHRARGARGGLU
3   ARGTHRLEUGLYALAPROALASERSERGLU
4   ARGSERALAALAGLYGLYARGGLYPROARG
5   GLYHISLYSARGLYSLEUASNGLUGLUASP
6   ALAALASERGLUSERSERARGGLUSERSER
7   ASNGLUASPGLUGLYSERSERSERGLUALA
8   ASPGLUMETALALYSALALEUGLUALAGLU
9   LEUASNASPLEUMET

Samples:

sample_1: FCP1, [U-100% 13C; U-100% 15N], 1 mM; sodium phosphate 20 mM; sodium chloride 100 mM; sodium azide 0.02%

sample_conditions_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D CCONHsample_1isotropicsample_conditions_1
2D CONsample_1isotropicsample_conditions_1
2D CACOsample_1isotropicsample_conditions_1
3D CBCACOsample_1isotropicsample_conditions_1
3D CCCOsample_1isotropicsample_conditions_1
3D CCCONsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 500 MHz

Related Database Links:

PDB
GB AAC64549 AAH52576 EAW66628
REF NP_001189433 NP_004706 XP_002828379 XP_003805250 XP_004059621
SP Q9Y5B0
AlphaFold Q9Y5B0

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks