BMRB Entry 16294

Name: 1H (NH, HA, HB, other) Chemical Shift Assignments of Peach Pru p 3, Non-Specific Lipid Transfer Protein (nsLTP) isolated from Peach fruit skin
Published: 2010-03-01

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16294

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H (NH, HA, HB, other) Chemical Shift Assignments of Peach Pru p 3, Non-Specific Lipid Transfer Protein (nsLTP) isolated from Peach fruit skin

Deposition date:

2009-05-08

Original release date:

2010-03-01

Authors:

Wijesinha-Bettoni, Ramani; Alexeev, Yuri; Johnson, Phil; Marsh, Justin; Sancho, Ana; Mackie, Alan; Shewry, Peter; Smith, Lorna; Mills, E. N

Citation:

Citation: Wijesinha-Bettoni, Ramani; Alexeev, Yuri; Johnson, Phil; Marsh, Justin; Sancho, Ana; Abdullah, Syed; Mackie, Alan; Shewry, Peter; Smith, Lorna; Mills, E. N Clare. "The structural characteristics of nonspecific lipid transfer proteins explain their resistance to gastroduodenal proteolysis." Biochemistry 49, 2130-2139 (2010).
PubMed: 20121231

Assembly members:

Assembly members:
Pru_p_3_(nsLTP), polymer, 91 residues, Formula weight is not available

Natural source:

Natural source: Common Name: peach Taxonomy ID: 3760 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Prunus persica

Experimental source:

Experimental source: Production method: purified from the natural source

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Pru_p_3_(nsLTP): ITCGQVSSSLAPCIPYVRGG GAVPPACCNGIRNVNNLART TPDRQAACNCLKQLSASVPG VNPNNAAALPGKCGVSIPYK ISASTNCATVK

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
1H chemical shifts	332

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	non specific lipid transfer protein from peach (Pru p 3)	1

Entities:

Entity 1, non specific lipid transfer protein from peach (Pru p 3) 91 residues - Formula weight is not available

1

ILE

THR

CYS

GLY

GLN

VAL

SER

LEU

2

ALA

PRO

CYS

ILE

PRO

TYR

VAL

ARG

GLY

3

GLY

ALA

VAL

PRO

ALA

CYS

ASN

GLY

4

ILE

ARG

ASN

VAL

ASN

LEU

ALA

ARG

THR

5

THR

PRO

ASP

ARG

GLN

ALA

CYS

ASN

CYS

6

LEU

LYS

GLN

LEU

SER

ALA

SER

VAL

PRO

GLY

7

VAL

ASN

PRO

ASN

ALA

LEU

PRO

8

GLY

LYS

CYS

GLY

VAL

SER

ILE

PRO

TYR

LYS

9

ILE

SER

ALA

SER

THR

ASN

CYS

ALA

THR

VAL

10

LYS

Samples:

sample_1: Pru p 3 (nsLTP)2.0 – 3.0 mM; H2O 95%; D2O 5%

sample_conditions_1: pH: 5.3; temperature: 310 K

sample_conditions_2: pH: 5.3; temperature: 298 K

sample_conditions_3: pH: 4; temperature: 298 K

sample_conditions_4: pH: 6.5; temperature: 293 K

sample_conditions_5: pH: 1.8; temperature: 310 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H COSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D DQF-COSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_2
2D 1H-1H COSY	sample_1	isotropic	sample_conditions_2
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_2
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_3
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_4
2D 1H-1H COSY	sample_1	isotropic	sample_conditions_4
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_4
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_5
2D 1H-1H COSY	sample_1	isotropic	sample_conditions_5
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_5

Software:

SPARKY vVersion 3.110, Goddard - data analysis

NMR spectrometers:

Home-built ?,AM 600 MHz

PDB	2ALG 2B5S
EMBL	CAB96876
GB	AAV40850 AAV64877 ACE80965 ADR66939 ADR66940
REF	XP_007206159
SP	P81402
AlphaFold	P81402