BMRB Entry 16273

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16273
MolProbity Validation Chart

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Title:
A PH domain within OCRL bridges clathrin mediated membrane trafficking to phosphoinositide metabolism
Deposition date:
2009-05-03
Original release date:
2009-06-25
Authors:
Mao, Yuxin; Hodsdon, Micheal; De Camilli, Pietro
Citation:

Citation: Mao, Yuxin; Balkin, Daniel; Zoncu, Roberto; Erdmann, Kai; Tomasini, Livia; Hu, Fenghua; Jin, Moonsoo; Hodsdon, Michael; De Camilli, Pietro. "A PH domain within OCRL bridges clathrin-mediated membrane trafficking to phosphoinositide metabolism"  EMBO J. 28, 1831-1842 (2009).
PubMed: 19536138

Assembly members:

Assembly members:
PH domain, polymer, 483 residues, 53980.047 Da.

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX6p-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PH domain: GPLGSMDQSVAIQETLVEGE YCVIAVQGVLCKGDSRQSRL LGLVRYRLENDAQEHALFLY THRRMAITGDDVSLDQIVPL SKDFMLEEVSPDGELYILGS DVTVQLNTAELKLVFQLPFG SHTRTFLQEVARACPGFDPE TRDPEFEWLSRHTCAEPDAE SGPLGSMDQSVAIQETLVEG EYCVIAVQGVLCKGDSRQSR LLGLVRYRLENDAQEHALFL YTHRRMAITGDDVSLDQIVP LSKDFMLEEVSPDGELYILG SDVTVQLNTAELKLVFQLPF GSHTRTFLQEVARACPGFDP ETRDPEFEWLSRHTCAEPDA ESGPLGSMDQSVAIQETLVE GEYCVIAVQGVLCKGDSRQS RLLGLVRYRLENDAQEHALF LYTHRRMAITGDDVSLDQIV PLSKDFMLEEVSPDGELYIL GSDVTVQLNTAELKLVFQLP FGSHTRTFLQEVARACPGFD PETRDPEFEWLSRHTCAEPD AES

Data sets:
Data typeCount
13C chemical shifts674
15N chemical shifts163
1H chemical shifts1113

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1PH domain1

Entities:

Entity 1, PH domain 483 residues - 53980.047 Da.

1   GLYPROLEUGLYSERMETASPGLNSERVAL
2   ALAILEGLNGLUTHRLEUVALGLUGLYGLU
3   TYRCYSVALILEALAVALGLNGLYVALLEU
4   CYSLYSGLYASPSERARGGLNSERARGLEU
5   LEUGLYLEUVALARGTYRARGLEUGLUASN
6   ASPALAGLNGLUHISALALEUPHELEUTYR
7   THRHISARGARGMETALAILETHRGLYASP
8   ASPVALSERLEUASPGLNILEVALPROLEU
9   SERLYSASPPHEMETLEUGLUGLUVALSER
10   PROASPGLYGLULEUTYRILELEUGLYSER
11   ASPVALTHRVALGLNLEUASNTHRALAGLU
12   LEULYSLEUVALPHEGLNLEUPROPHEGLY
13   SERHISTHRARGTHRPHELEUGLNGLUVAL
14   ALAARGALACYSPROGLYPHEASPPROGLU
15   THRARGASPPROGLUPHEGLUTRPLEUSER
16   ARGHISTHRCYSALAGLUPROASPALAGLU
17   SERGLYPROLEUGLYSERMETASPGLNSER
18   VALALAILEGLNGLUTHRLEUVALGLUGLY
19   GLUTYRCYSVALILEALAVALGLNGLYVAL
20   LEUCYSLYSGLYASPSERARGGLNSERARG
21   LEULEUGLYLEUVALARGTYRARGLEUGLU
22   ASNASPALAGLNGLUHISALALEUPHELEU
23   TYRTHRHISARGARGMETALAILETHRGLY
24   ASPASPVALSERLEUASPGLNILEVALPRO
25   LEUSERLYSASPPHEMETLEUGLUGLUVAL
26   SERPROASPGLYGLULEUTYRILELEUGLY
27   SERASPVALTHRVALGLNLEUASNTHRALA
28   GLULEULYSLEUVALPHEGLNLEUPROPHE
29   GLYSERHISTHRARGTHRPHELEUGLNGLU
30   VALALAARGALACYSPROGLYPHEASPPRO
31   GLUTHRARGASPPROGLUPHEGLUTRPLEU
32   SERARGHISTHRCYSALAGLUPROASPALA
33   GLUSERGLYPROLEUGLYSERMETASPGLN
34   SERVALALAILEGLNGLUTHRLEUVALGLU
35   GLYGLUTYRCYSVALILEALAVALGLNGLY
36   VALLEUCYSLYSGLYASPSERARGGLNSER
37   ARGLEULEUGLYLEUVALARGTYRARGLEU
38   GLUASNASPALAGLNGLUHISALALEUPHE
39   LEUTYRTHRHISARGARGMETALAILETHR
40   GLYASPASPVALSERLEUASPGLNILEVAL
41   PROLEUSERLYSASPPHEMETLEUGLUGLU
42   VALSERPROASPGLYGLULEUTYRILELEU
43   GLYSERASPVALTHRVALGLNLEUASNTHR
44   ALAGLULEULYSLEUVALPHEGLNLEUPRO
45   PHEGLYSERHISTHRARGTHRPHELEUGLN
46   GLUVALALAARGALACYSPROGLYPHEASP
47   PROGLUTHRARGASPPROGLUPHEGLUTRP
48   LEUSERARGHISTHRCYSALAGLUPROASP
49   ALAGLUSER

Samples:

sample_1: entity mM; potassium phosphate 20 mM

sample_conditions_1: pH: 6.4; pressure: 1.0 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - peak picking

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement

NMR spectrometers:

  • Varian INOVA 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks